Proteintech, 83725-5-RR, MICA/MICB Recombinant monoclonal antibody

Size: 20ul / 100ul The MICA/MICB (83725-5-RR) by Proteintech is a Recombinant antibody targeting MICA/MICB in WB, ELISA applications with reactivity to human samples 83725-5-RR targets MICA/MICB in WB, ELISA applications and shows reactivity with human samples. Tested Applications Positive WB detected in: K-562 cells, HeLa cells Recommended dilution Western Blot (WB): WB : 1:2000-1:16000 Background Information Human MHC class I chain-related genes located within the HLA class I region of chromosome 6 encode MHC class I chain-related A and B (MICA and MICB) (PMID: 11429322). MICA and MICB are stress-inducible surface molecules that are not associated with β2-microglobulin and do not present peptides (PMID: 9497295). They are expressed in intestinal epithelium and many epithelial tumors (PMID: 10359807). MICA and MICB are ligands for NKG2D which is an activating receptor that is expressed on most natural killer (NK) cells, CD8 αβ T cells, and γδ T cells (PMID: 10426993; 11491531). This antibody recognizes both MICA and MICB. Specification Tested Reactivity: human Host / Isotype: Rabbit / IgG Class: Recombinant Type: Antibody Immunogen: Recombinant protein Predict reactive species Full Name: MHC class I polypeptide-related sequence A Calculated Molecular Weight: 43 kDa Observed Molecular Weight: 50-60 kDa GenBank Accession Number: / Gene Symbol: MICA Gene ID (NCBI): 100507436 RRID: AB_3671325 Conjugate: Unconjugated Form: Liquid Purification Method: Protein A purfication UNIPROT ID: Q29983 Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20 o C storage. 20ul sizes contain 0.1% BSA.