Proteintech, 83728-4-RR, APOE Recombinant monoclonal antibody

Size: 20ul / 100ul The Apoe (83728-4-RR) by Proteintech is a Recombinant antibody targeting Apoe in WB, IHC, IF/ICC, ELISA applications with reactivity to mouse, rat samples 83728-4-RR targets Apoe in WB, IHC, IF/ICC, ELISA applications and shows reactivity with mouse, rat samples. Tested Applications Positive WB detected in: mouse liver tissue, mouse serum, mouse brain tissue Positive IHC detected in: mouse brain tissue, mouse liver tissue, rat cerebellum tissueNote: suggested antigen retrieval withTE buffer pH 9.0;(*) Alternatively, antigen retrieval may be performed withcitrate buffer pH 6.0 Positive IF/ICC detected in: HepG2 cells Recommended dilution Western Blot (WB): WB : 1:5000-1:50000 Immunohistochemistry (IHC): IHC : 1:300-1:1200 Immunofluorescence (IF)/ICC: IF/ICC : 1:125-1:500 Background Information Apolipoprotein E (Apoe) is a core component of plasma lipoproteins that participates in diverse biological processes, including plasma lipoprotein metabolism, intracellular cholesterol utilization, cell growth, immunoregulation, and neuronal growth and repair (PMID: 17854398). Mouse apoE, like apoE4, contains the equivalent of Arg-112 and Glu-255, but lacks the critical Arg-61 equivalent (it contains Thr-61). Thus, mouse apoE does not display apoE4 domain interaction (PMID: 11553788). Specification Tested Reactivity: mouse, rat Host / Isotype: Rabbit / IgG Class: Recombinant Type: Antibody Immunogen: Fusion Protein Predict reactive species Full Name: Apolipoprotein E Calculated Molecular Weight: 36kDa Observed Molecular Weight: 36 kDa GenBank Accession Number: NM_001305819.1 Gene Symbol: Apoe Gene ID (NCBI): 11816 RRID: AB_3671326 Conjugate: Unconjugated Form: Liquid Purification Method: Protein A purfication UNIPROT ID: P08226 Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20 o C storage. 20ul sizes contain 0.1% BSA.