Proteintech, 83809-1-RR, TP53BP1-Specific Recombinant monoclonal antibody

Size: 20ul / 100ul The TP53BP1-Specific (83809-1-RR) by Proteintech is a Recombinant antibody targeting TP53BP1-Specific in WB, IHC, IF/ICC, FC (Intra), ELISA applications with reactivity to human samples 83809-1-RR targets TP53BP1-Specific in WB, IHC, IF/ICC, FC (Intra), ELISA applications and shows reactivity with human samples. Tested Applications Positive WB detected in: HeLa cells, Jurkat cells, K-562 cells Positive IHC detected in: human ovary cancer tissueNote: suggested antigen retrieval withTE buffer pH 9.0;(*) Alternatively, antigen retrieval may be performed withcitrate buffer pH 6.0 Positive IF/ICC detected in: HeLa cells Positive FC (Intra) detected in: HeLa cells Recommended dilution Western Blot (WB): WB : 1:5000-1:50000 Immunohistochemistry (IHC): IHC : 1:200-1:800 Immunofluorescence (IF)/ICC: IF/ICC : 1:125-1:500 Flow Cytometry (FC) (INTRA): FC (INTRA) : 0.25 ug per 10^6 cells in a 100 µl suspension Background Information TP53BP1, also named as p53BP1, p202, 53BP1 and FLJ41424, may have a role in checkpoint signaling during mitosis. TP53BP1 enhances TP53-mediated transcriptional activation. TP53BP1 plays a role in the response to DNA damage. Specification Tested Reactivity: human Host / Isotype: Rabbit / IgG Class: Recombinant Type: Antibody Immunogen: Peptide Predict reactive species Full Name: tumor protein p53 binding protein 1 Calculated Molecular Weight: 214 kDa Observed Molecular Weight: 450 kDa GenBank Accession Number: NM_005657 Gene Symbol: TP53BP1 Gene ID (NCBI): 7158 RRID: AB_3671397 Conjugate: Unconjugated Form: Liquid Purification Method: Protein A purfication UNIPROT ID: Q12888 Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20 o C storage. 20ul sizes contain 0.1% BSA.