Proteintech, 83811-1-RR, Phospho-Histone H2B (Ser14) Recombinant monoclonal antibody

Size: 20ul / 100ul The Phospho-Histone H2B (Ser14) (83811-1-RR) by Proteintech is a Recombinant antibody targeting Phospho-Histone H2B (Ser14) in WB, ELISA applications with reactivity to human, mouse samples 83811-1-RR targets Phospho-Histone H2B (Ser14) in WB, ELISA applications and shows reactivity with human, mouse samples. Tested Applications Positive WB detected in: PMA treated NIH/3T3 cells Recommended dilution Western Blot (WB): WB : 1:500-1:2000 Background Information Histones are nuclear proteins that are classified into five major protein groups: histones H2A, H2B, H3, and H4 are known as the core histones. Post-translationally modified H2B proteins can modulate the nucleosome/chromatin structure or DNA accessibility to affect the transcriptional pathways linked to embryonic development and cell differentiation. In addition, MST1 is known to phosphorylate histone H2B in vitro and has recently been shown to directly phosphorylate histone H2B at Ser14 in vivo. (PMID: 17548476) Specification Tested Reactivity: human, mouse Host / Isotype: Rabbit / IgG Class: Recombinant Type: Antibody Immunogen: Peptide Predict reactive species Full Name: histone cluster 2, H2be Calculated Molecular Weight: 14 kDa Observed Molecular Weight: 14-17 kDa GenBank Accession Number: BC005827 Gene Symbol: Histone H2B Gene ID (NCBI): 8349 RRID: AB_3671399 Conjugate: Unconjugated Form: Liquid Purification Method: Protein A purfication UNIPROT ID: Q16778 Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20 o C storage. 20ul sizes contain 0.1% BSA.