Proteintech, 83851-6-RR, PTPN12 Recombinant monoclonal antibody

Size: 20ul / 100ul The PTPN12 (83851-6-RR) by Proteintech is a Recombinant antibody targeting PTPN12 in WB, IHC, IF/ICC, ELISA applications with reactivity to human samples 83851-6-RR targets PTPN12 in WB, IHC, IF/ICC, ELISA applications and shows reactivity with human samples. Tested Applications Positive WB detected in: HeLa cells, HepG2 cells, MCF-7 cells, HEK-293T cells, A549 cells Positive IHC detected in: human ovary cancer tissueNote: suggested antigen retrieval withTE buffer pH 9.0;(*) Alternatively, antigen retrieval may be performed withcitrate buffer pH 6.0 Positive IF/ICC detected in: A431 cells, U-251 cells Recommended dilution Western Blot (WB): WB : 1:5000-1:50000 Immunohistochemistry (IHC): IHC : 1:500-1:2000 Immunofluorescence (IF)/ICC: IF/ICC : 1:200-1:800 Background Information PTPN12 (Tyrosine-protein phosphatase non-receptor type 12), also referred to as PTP-PEST (PTP-proline, glutamic acid, serine, and threonine-rich), is a cytoplasmic PTP expressed in a wide spectrum of cell types. PTPN12 has been identified as a potent tumor suppressor in human cancers and a critical regulator of cell adhesion and migration (PMID: 26391955, 29743287). PTPN12 is phosphorylated on serine and threonine residues and is rich in proline (P), glutamate (E), serine (S), and threonine (T) residues. Western blot analysis detected PTPN12 at an apparent molecular mass of 110 kDa. Specification Tested Reactivity: human Host / Isotype: Rabbit / IgG Class: Recombinant Type: Antibody Immunogen: CatNo: Ag35382 Product name: Recombinant human PTPN12 protein Source: e coli. -derived, PGEX-4T Tag: GST Domain: 603-780 aa of NM_002835.3 Sequence: SDDSDSDERNSDGAVTQNKTNISTASATVSAATSTESISTRKVLPMSIARHNIAGTTHSGAEKDVDVSEDSPPPLPERTPESFVLASEHNTPVRSEWSELQSQERSEQKKSEGLITSENEKCDHPAGGIHYEMCIECPPTFSDKREQISENPTEATDIGFGNRCGKPKGPRDPPSEWT Predict reactive species Full Name: protein tyrosine phosphatase, non-receptor type 12 Calculated Molecular Weight: 88kDa Observed Molecular Weight: 110-125 kDa GenBank Accession Number: NM_002835.3 Gene Symbol: PTPN12 Gene ID (NCBI): 5782 RRID: AB_3671436 Conjugate: Unconjugated Form: Liquid Purification Method: Protein A purfication UNIPROT ID: Q05209 Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20 o C storage. 20ul sizes contain 0.1% BSA.