Proteintech, 83878-5-RR, AES Recombinant monoclonal antibody

Size: 20ul / 100ul The AES (83878-5-RR) by Proteintech is a Recombinant antibody targeting AES in WB, ELISA applications with reactivity to human, mouse, rat samples 83878-5-RR targets AES in WB, ELISA applications and shows reactivity with human, mouse, rat samples. Tested Applications Positive WB detected in: HEK-293 cells, K-562 cells, DU 145 cells, MDA-MB-231 cells, NIH/3T3 cells, mouse skeletal muscle tissue, rat skeletal muscle tissue Recommended dilution Western Blot (WB): WB : 1:5000-1:50000 Background Information Amino-terminal enhancer of split (AES), belongs to the groucho/TLE family of proteins, can function as a homooligomer or as a heteroologimer with other family members in order to forcefully repress the expression of other family member genes. AES can repress NFkB-regulated gene expression and is believed to have a significant role in initiating and maintaining cell differentiation events. AES has been found to suppress local invasion and intravasation through inhibition of the Notch pathway, leading to preventing the metastatic spread of endogenous tumors(PMID: 33754069). Specification Tested Reactivity: human, mouse, rat Host / Isotype: Rabbit / IgG Class: Recombinant Type: Antibody Immunogen: CatNo: Ag21021 Product name: Recombinant human AES protein Source: e coli. -derived, PGEX-4T Tag: GST Domain: 1-81 aa of BC113735 Sequence: MCHKNGFPQEGGITAAFLQKRKLRLSKNHRPARAKVTEHVRGTRPGRATAGPAASTRAAGSLFFDRWGNRGPAGCRGSSHL Predict reactive species Full Name: amino-terminal enhancer of split Calculated Molecular Weight: 264 aa, 29 kDa Observed Molecular Weight: 22-29 kDa GenBank Accession Number: BC113735 Gene Symbol: AES Gene ID (NCBI): 166 RRID: AB_3671462 Conjugate: Unconjugated Form: Liquid Purification Method: Protein A purfication UNIPROT ID: Q08117 Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20 o C storage. 20ul sizes contain 0.1% BSA.