Proteintech, 83898-1-RR, PLEC Recombinant monoclonal antibody

Size: 20ul / 100ul The PLEC (83898-1-RR) by Proteintech is a Recombinant antibody targeting PLEC in WB, IF/ICC, ELISA applications with reactivity to human samples 83898-1-RR targets PLEC in WB, IF/ICC, ELISA applications and shows reactivity with human samples. Tested Applications Positive WB detected in: HaCaT cells, A431 cells, HEK-293 cells Positive IF/ICC detected in: A431 cells Recommended dilution Western Blot (WB): WB : 1:1000-1:8000 Immunofluorescence (IF)/ICC: IF/ICC : 1:250-1:1000 Background Information Plectin is a large (≥500-kDa) protein that is normally expressed in various tissues including skin, muscle, and brain. It binds to a number of cytoskeletal proteins including microtubules and intermediate filaments and is involved in establishment and dynamic modulation of the cytoskeletal network. In normal cells, it plays a crucial role in cytoskeleton network organization. In keratinocytes, plectin is concentrated at the basal surface, where it links intermediate filaments to the cytoplasmic domain of transmembrane glycoproteins such as integrin beta-4. (PMID: 24218614, PMID: 28281696, PMID: 23750011) Specification Tested Reactivity: human Host / Isotype: Rabbit / IgG Class: Recombinant Type: Antibody Immunogen: CatNo: Ag29418 Product name: Recombinant human PLEC protein Source: e coli. -derived, PGEX-4T Tag: GST Domain: 1475-1768 aa of NM_201380 Sequence: SETLRRMEEEERLAEQQRAEERERLAEVEAALEKQRQLAEAHAQAKAQAEREAKELQQRMQEEVVRREEAAVDAQQQKRSIQEELQQLRQSSEAEIQAKARQAEAAERSRLRIEEEIRVVRLQLEATERQRGGAEGELQALRARAEEAEAQKRQAQEEAERLRRQVQDESQRKRQAEVELASRVKAEAEAAREKQRALQALEELRLQAEEAERRLRQAEVERARQVQVALETAQRSAEAELQSKRASFAEKTAQLERSLQEEHVAVAQLREEAERRAQQQAEAERAREEAEREL Predict reactive species Full Name: plectin 1, intermediate filament binding protein 500kDa Calculated Molecular Weight: 532 kDa Observed Molecular Weight: 510 kDa GenBank Accession Number: NM_201380 Gene Symbol: Plectin Gene ID (NCBI): 5339 RRID: AB_3671479 Conjugate: Unconjugated Form: Liquid Purification Method: Protein A purification UNIPROT ID: Q15149 Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20 o C storage. 20ul sizes contain 0.1% BSA.