Proteintech, 84001-1-RR, PARP4 Recombinant monoclonal antibody

Size: 20ul / 100ul The PARP4 (84001-1-RR) by Proteintech is a Recombinant antibody targeting PARP4 in WB, IHC, IF/ICC, FC (Intra), ELISA applications with reactivity to human samples 84001-1-RR targets PARP4 in WB, IHC, IF/ICC, FC (Intra), ELISA applications and shows reactivity with human samples. Tested Applications Positive WB detected in: A549 cells, MDA-MB-231 cells, HepG2 cells, U2OS cells, HeLa cells Positive IHC detected in: human stomach cancer tissueNote: suggested antigen retrieval withTE buffer pH 9.0;(*) Alternatively, antigen retrieval may be performed withcitrate buffer pH 6.0 Positive IF/ICC detected in: HeLa cells Positive FC (Intra) detected in: HeLa cells, U-2 OS Recommended dilution Western Blot (WB): WB : 1:5000-1:50000 Immunohistochemistry (IHC): IHC : 1:500-1:2000 Immunofluorescence (IF)/ICC: IF/ICC : 1:50-1:500 Flow Cytometry (FC) (INTRA): FC (INTRA) : 0.25 ug per 10^6 cells in a 100 µl suspension Background Information PARP4, also named as ADPRTL1, KIAA0177, PARPL, VPARP and PH5P, catalyzes the reaction: NAD+ + (ADP-D-ribosyl)(n)-acceptor = nicotinamide + (ADP-D-ribosyl)(n+1)-acceptor. Specification Tested Reactivity: human Host / Isotype: Rabbit / IgG Class: Recombinant Type: Antibody Immunogen: Peptide Predict reactive species Full Name: poly (ADP-ribose) polymerase family, member 4 Calculated Molecular Weight: 193 kDa Observed Molecular Weight: 193 kDa GenBank Accession Number: NM_006437 Gene Symbol: PARP4 Gene ID (NCBI): 143 RRID: AB_3671573 Conjugate: Unconjugated Form: Liquid Purification Method: Protein A purfication UNIPROT ID: Q9UKK3 Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20 o C storage. 20ul sizes contain 0.1% BSA.