Proteintech, 84030-5-RR, PEG3 Recombinant monoclonal antibody

Size: 20ul / 100ul The PEG3 (84030-5-RR) by Proteintech is a Recombinant antibody targeting PEG3 in WB, IHC, IF/ICC, FC (Intra), ELISA applications with reactivity to human samples 84030-5-RR targets PEG3 in WB, IHC, IF/ICC, FC (Intra), ELISA applications and shows reactivity with human samples. Tested Applications Positive WB detected in: TT cells, HepG2 cells Positive IHC detected in: mouse brain tissueNote: suggested antigen retrieval withTE buffer pH 9.0;(*) Alternatively, antigen retrieval may be performed withcitrate buffer pH 6.0 Positive IF/ICC detected in: HepG2 cells Positive FC (Intra) detected in: HepG2 cells Recommended dilution Western Blot (WB): WB : 1:2000-1:10000 Immunohistochemistry (IHC): IHC : 1:200-1:800 Immunofluorescence (IF)/ICC: IF/ICC : 1:150-1:600 Flow Cytometry (FC) (INTRA): FC (INTRA) : 0.25 ug per 10^6 cells in a 100 µl suspension Background Information Paternally expressed 3 (PEG3) was responsible for Kras/Erk- and mTOR-driven HCC (hepatocellular carcinoma) (PMID: 36451866). PEG3 can regulate the physiological processes related to energy homeostasis found in multiple tissues, such as the brain, testis, ovary, and placenta (PMID: 32729618). Epigenetic silencing and loss of imprinting of PEG3 are implicated in glioma tumorigenesis (PMID: 32231463). Specification Tested Reactivity: human Host / Isotype: Rabbit / IgG Class: Recombinant Type: Antibody Immunogen: CatNo: Ag20132 Product name: Recombinant human PEG3 protein Source: e coli. -derived, PGEX-4T Tag: GST Domain: 1116-1464 aa of BC150272 Sequence: LNEHMRLHREDDLLEQSQMAEEAIIPGLALTEFQRSQTEERLFECAVCGESFVNPAELADHVTVHKNEPYEYGSSYTHTSFLTEPLKGAIPFYECKDCGKSFIHSTVLTKHKELHLEEEEEDEAAAAAAAAAQEVEANVHVPQVVLRIQGLNVEAAEPEVEAAEPEVEAAEPEVEAAEPNGEAEGPDGEAAEPIGEAGQPNGEAEQPNGDADEPDGAGIEDPEERAEEPEGKAEEPEGDADEPDGVGIEDPEEGEDQEIQVEEPYYDCHECTETFTSSTAFSEHLKTHASMIIFEPANAFGECSGYIERASTSTGGANQADEKYFKCDVCGQLFNDRLSLARHQNTHTG Predict reactive species Full Name: paternally expressed 3 Calculated Molecular Weight: 1588 aa, 181 kDa Observed Molecular Weight: 200-220 kDa GenBank Accession Number: BC150272 Gene Symbol: PEG3 Gene ID (NCBI): 5178 RRID: AB_3671596 Conjugate: Unconjugated Form: Liquid Purification Method: Protein A purfication UNIPROT ID: Q9GZU2 Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20 o C storage. 20ul sizes contain 0.1% BSA.