Proteintech, 84049-1-RR, TOP2B Recombinant monoclonal antibody

Size: 20ul / 100ul The TOP2B (84049-1-RR) by Proteintech is a Recombinant antibody targeting TOP2B in WB, IF/ICC, ELISA applications with reactivity to human samples 84049-1-RR targets TOP2B in WB, IF/ICC, ELISA applications and shows reactivity with human samples. Tested Applications Positive WB detected in: Jurkat cells, HepG2 cells, HeLa cells, K-562 cells, HEK-293T cells Positive IF/ICC detected in: HeLa cells Recommended dilution Western Blot (WB): WB : 1:5000-1:50000 Immunofluorescence (IF)/ICC: IF/ICC : 1:200-1:800 Background Information TOP2B belongs to the type II topoisomerase family. TOP2B control topological states of DNA by transient breakage and subsequent rejoining of DNA strands. TOP2B makes double-strand breaks. It is indirectly ivolved in vitamin D-coupled transcription regulation via its association with the WINAC complex, a chromatin-remodeling complex recruited by vitamin D receptor (VDR), which is required for the ligand-bound VDR-mediated transrepression of the CYP27B1 gene. Specification Tested Reactivity: human Host / Isotype: Rabbit / IgG Class: Recombinant Type: Antibody Immunogen: Peptide Predict reactive species Full Name: topoisomerase (DNA) II beta 180kDa Calculated Molecular Weight: 183 kDa Observed Molecular Weight: 180-190 kDa GenBank Accession Number: NM_001068 Gene Symbol: TOP2B Gene ID (NCBI): 7155 RRID: AB_3671617 Conjugate: Unconjugated Form: Liquid Purification Method: Protein A purfication UNIPROT ID: Q02880 Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20 o C storage. 20ul sizes contain 0.1% BSA.