Proteintech, 84080-3-RR, SEMA3B Recombinant monoclonal antibody

Size: 20ul / 100ul The SEMA3B (84080-3-RR) by Proteintech is a Recombinant antibody targeting SEMA3B in WB, IF/ICC, ELISA applications with reactivity to human samples 84080-3-RR targets SEMA3B in WB, IF/ICC, ELISA applications and shows reactivity with human samples. Tested Applications Positive WB detected in: HeLa cells, HepG2 cells, MCF-7 cells, U-251 cells, SK-N-SH cells Positive IF/ICC detected in: U-251 cells Recommended dilution Western Blot (WB): WB : 1:1000-1:4000 Immunofluorescence (IF)/ICC: IF/ICC : 1:250-1:1000 Background Information SEMA3B is a class 3 semaphorin/collapsin family member containing 749 highly conserved, NH2 terminal, semaphorin amino acids. SEMA3B is characterized as a strong suppressing factor of the proliferation of cancerous cells and also by its anti-angiogenic effect. SEMA3B has been found in lung cancer, ovarian cancer, hepatocellular carcinoma, and cholangiocarcinoma. SEMA3B is expressed abundantly but differentially in various neural and nonneural tissues. The calculated molecular weight of SEMA3B is 83 kDa (PMID: 30914894, PMID: 32410560). Specification Tested Reactivity: human Host / Isotype: Rabbit / IgG Class: Recombinant Type: Antibody Immunogen: CatNo: Ag25163 Product name: Recombinant human SEMA3B protein Source: e coli. -derived, PGEX-4T Tag: GST Domain: 450-629 aa of BC009113 Sequence: TLCSGDSSRPALLEHKVFGVEGSSAFLECEPRSLQARVEWTFQRAGVTAHTQVLAEERTERTARGLLLRRLRRRDSGVYLCAAVEQGFTQPLRRLSLHVLSATQAERLARAEEAAPAAPPGPKLWYRDFLQLVEPGGGGSANSLRMCRPQPALQSLPLESRRKGRNRRTHAPEPRAERGP Predict reactive species Full Name: sema domain, immunoglobulin domain (Ig), short basic domain, secreted, (semaphorin) 3B Observed Molecular Weight: 83 kDa GenBank Accession Number: BC009113 Gene Symbol: SEMA3B Gene ID (NCBI): 7869 RRID: AB_3671648 Conjugate: Unconjugated Form: Liquid Purification Method: Protein A purfication UNIPROT ID: Q13214 Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20 o C storage. 20ul sizes contain 0.1% BSA.