Proteintech, 84097-6-RR, RNASEH2B Recombinant monoclonal antibody

Size: 20ul / 100ul The RNASEH2B (84097-6-RR) by Proteintech is a Recombinant antibody targeting RNASEH2B in WB, IF/ICC, ELISA applications with reactivity to human samples 84097-6-RR targets RNASEH2B in WB, IF/ICC, ELISA applications and shows reactivity with human samples. Tested Applications Positive WB detected in: MCF-7 cells, HeLa cells, Jurkat cells, U-937 cells, THP-1 cells Positive IF/ICC detected in: HeLa cells Recommended dilution Western Blot (WB): WB : 1:1000-1:6000 Immunofluorescence (IF)/ICC: IF/ICC : 1:200-1:800 Background Information Ribonuclease H2 is the major nuclear enzyme degrading cellular RNA/DNA hybrids in eukaryotes and the sole nuclease known to be able to hydrolyze ribonucleotides misincorporated during genomic replication. RNASEH2B and RNASEH2C are the non-catalytic subunits of RNase H2 complex. Defects in RNASEH2B are a cause of Aicardi-Goutieres syndrome type 2 (AGS2), a genetically heterogeneous disease with clinical features as thrombocytopenia, hepatosplenomegaly and elevated hepatic transaminases along with intermittent fever may erroneously suggest an infective process. Specification Tested Reactivity: human Host / Isotype: Rabbit / IgG Class: Recombinant Type: Antibody Immunogen: CatNo: Ag33669 Product name: Recombinant human RNASEH2B protein Source: e coli. -derived, PGEX-4T Tag: GST Domain: 1-331 aa of BC036744 Sequence: MAAGVDCGDGVGARQHVFLVSEYLKDASKKMKNGLMFVKLVNPCSGEGAIYLFNMCLQQLFEVKVFKEKHHSWFINQSVQSGGLLHFATPVDPLFLLLHYLIKADKEGKFQPLDQVVVDNVFPNCILLLKLPGLEKLLHHVTEEKGNPEIDNKKYYKYSKEKTLKWLEKKVNQTVAALKTNNVNVSSRVQSTAFFSGDQASTDKEKDYIRYAHGLISDYIPKELSDDLSKYLKLPEPSASLPNPPSKKIKLSDEPVEAKEDYTKFNTKDLKTEKKNSKMTAAQKALAKVDKSGMKSIDTFFGVKKKLERFETLKIKSSKNICFLHVSVCPS* Predict reactive species Full Name: ribonuclease H2, subunit B Calculated Molecular Weight: 331 aa, 37 kDa Observed Molecular Weight: 35 kDa GenBank Accession Number: BC036744 Gene Symbol: RNASEH2B Gene ID (NCBI): 79621 RRID: AB_3671663 Conjugate: Unconjugated Form: Liquid Purification Method: Protein A purfication UNIPROT ID: Q5TBB1 Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20 o C storage. 20ul sizes contain 0.1% BSA.