Proteintech, 84235-4-RR, CD39/ENTPD1 Recombinant monoclonal antibody

Size: 20ul / 100ul The CD39/ENTPD1 (84235-4-RR) by Proteintech is a Recombinant antibody targeting CD39/ENTPD1 in WB, IHC, ELISA applications with reactivity to human samples 84235-4-RR targets CD39/ENTPD1 in WB, IHC, ELISA applications and shows reactivity with human samples. Tested Applications Positive WB detected in: human placenta tissue Positive IHC detected in: human tonsillitis tissue, human placenta tissueNote: suggested antigen retrieval withTE buffer pH 9.0;(*) Alternatively, antigen retrieval may be performed withcitrate buffer pH 6.0 Recommended dilution Western Blot (WB): WB : 1:5000-1:50000 Immunohistochemistry (IHC): IHC : 1:500-1:2000 Background Information ENTPD1(Ectonucleoside triphosphate diphosphohydrolase 1) is also named CD39, NTPDase 1, and Ecto-ATPDase 1 and belongs to the GDA1/CD39 NTPase family. In the nervous system, it can hydrolyze ATP and other nucleotides to regulate purinergic neurotransmission. It is a noncovalent tetrameric protein and detergent inhibition of enzymatic activity is caused by dissociation of ectoapyrase tetramers into monomers(PMID: 9733785 ). And it is a 70- to 100-kDa protein that is heavily N-glycosylated(PMID:7930580). This protein has 6 glycosylation sites and 3 isoforms(58 kDa, 59 kDa, and 34 kDa) produced by alternative splicing. Specification Tested Reactivity: human Host / Isotype: Rabbit / IgG Class: Recombinant Type: Antibody Immunogen: Recombinant Protein Predict reactive species Full Name: ectonucleoside triphosphate diphosphohydrolase 1 Calculated Molecular Weight: 58 kDa Observed Molecular Weight: 75 kDa GenBank Accession Number: BC047664 Gene Symbol: CD39 Gene ID (NCBI): 953 RRID: AB_3671788 Conjugate: Unconjugated Form: Liquid Purification Method: Protein A purfication UNIPROT ID: P49961 Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20 o C storage. 20ul sizes contain 0.1% BSA.