Proteintech, 84236-4-RR, CD79a Recombinant monoclonal antibody

Size: 20ul / 100ul The CD79a (84236-4-RR) by Proteintech is a Recombinant antibody targeting CD79a in WB, IHC, ELISA applications with reactivity to human samples 84236-4-RR targets CD79a in WB, IHC, ELISA applications and shows reactivity with human samples. Tested Applications Positive WB detected in: Daudi cells, Ramos cells, Raji cells Positive IHC detected in: human tonsillitis tissueNote: suggested antigen retrieval withTE buffer pH 9.0;(*) Alternatively, antigen retrieval may be performed withcitrate buffer pH 6.0 Recommended dilution Western Blot (WB): WB : 1:5000-1:50000 Immunohistochemistry (IHC): IHC : 1:2000-1:8000 Background Information CD79a, also named as B-cell antigen receptor complex-associated protein alpha chain or MB-1 membrane glycoprotein, is a 226 amino acid protein, which contains one ITAM domain and one Ig-like C2-type (immunoglobulin-like) domain. CD79A is expressed in B cells and localizes in the cell membrane. CD79A is required in cooperation with CD79B for initiation of the signal transduction cascade activated by binding of antigen to the B-cell antigen receptor complex (BCR) which leads to internalization of the complex, trafficking to late endosomes and antigen presentation. CD79A is also required for BCR surface expression and for efficient differentiation of pro- and pre-B-cells. Specification Tested Reactivity: human Host / Isotype: Rabbit / IgG Class: Recombinant Type: Antibody Immunogen: Recombinant Protein Predict reactive species Full Name: CD79a molecule, immunoglobulin-associated alpha Calculated Molecular Weight: 226 aa, 25 kDa Observed Molecular Weight: 40-50 kDa GenBank Accession Number: BC113733 Gene Symbol: CD79A Gene ID (NCBI): 973 RRID: AB_3671789 Conjugate: Unconjugated Form: Liquid Purification Method: Protein A purfication UNIPROT ID: P11912 Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20 o C storage. 20ul sizes contain 0.1% BSA.