Proteintech, 84256-1-RR, IL3RA/CD123 Recombinant monoclonal antibody

Size: 20ul / 100ul The IL3RA/CD123 (84256-1-RR) by Proteintech is a Recombinant antibody targeting IL3RA/CD123 in IHC, ELISA applications with reactivity to human samples 84256-1-RR targets IL3RA/CD123 in IHC, ELISA applications and shows reactivity with human samples. Tested Applications Positive IHC detected in: human tonsil tissueNote: suggested antigen retrieval withTE buffer pH 9.0;(*) Alternatively, antigen retrieval may be performed withcitrate buffer pH 6.0 Recommended dilution Immunohistochemistry (IHC): IHC : 1:250-1:1000 Background Information Interleukin-3 receptor subunit alpha (IL3RA), also known as CD123, belongs to the cytokine receptor family. It is a glycoprotein containing an extracellular domain, involving a predicted Ig-like domain, two FnIII domains, a transmembrane domain and an intracellular domain (PMID: 24513123). IL3RA is expressed on hematopoietic progenitor cells, plasmacytoid dendritic cells, basophils, monocytes, some B cells, and in a variety of hematologic malignancies (PMID: 10527461; 34855461). IL-3 initially binds to IL3RA and subsequently recruits the beta chain (CD131) to form the high-affinity receptor, resulting in the activation of a series of signaling pathways including the JAK/STAT, Ras-MAPK, and phosphatidylinositol 3-kinase pathways (PMID: 29296749). IL-3R signaling regulates the proliferation, survival, and differentiation of hematopoietic cells. Specification Tested Reactivity: human Host / Isotype: Rabbit / IgG Class: Recombinant Type: Antibody Immunogen: Recombinant Protein Predict reactive species Full Name: interleukin 3 receptor, alpha (low affinity) Calculated Molecular Weight: 378 aa, 43 kDa GenBank Accession Number: BC035407 Gene Symbol: CD123 Gene ID (NCBI): 3563 ENSEMBL Gene ID: ENSG00000185291 Conjugate: Unconjugated Form: Liquid Purification Method: Protein A purification UNIPROT ID: P26951 Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20 o C storage. 20ul sizes contain 0.1% BSA.