Proteintech, 84257-1-RR, METTL2 Recombinant monoclonal antibody

Size: 20ul / 100ul The METTL2 (84257-1-RR) by Proteintech is a Recombinant antibody targeting METTL2 in WB, IF/ICC, ELISA applications with reactivity to human samples 84257-1-RR targets METTL2 in WB, IF/ICC, ELISA applications and shows reactivity with human samples. Tested Applications Positive WB detected in: A549 cells, HeLa cells, MCF-7 cells, SH-SY5Y cells, PC-3 cells Positive IF/ICC detected in: MCF-7 cells Recommended dilution Western Blot (WB): WB : 1:2000-1:10000 Immunofluorescence (IF)/ICC: IF/ICC : 1:125-1:500 Background Information METTL2A and METTL2B are required for the threonyl and argininyl tRNA methylation. METTL2 methyltransferase is responsible for 3-methylcytosine modification of arginine tRNAs in mammals. METTL2A and METTL2B are in higher homology 99%. This antibody detects both METTL2A and METTL2B. Specification Tested Reactivity: human Host / Isotype: Rabbit / IgG Class: Recombinant Type: Antibody Immunogen: CatNo: Ag10410 Product name: Recombinant human METTL2B protein Source: e coli. -derived, PGEX-4T Tag: GST Domain: 1-313 aa of BC107586 Sequence: MPVDYEINAHKYWNDFYKIHENGFFKDRHWLFTEFPELAPSQNQNHLKDWFLENKSEVCECRNNEDGPGLIMEEQHKCSSKSLEHKTQTPPVEENVTQKISDLEICADEFPGSSATYRILEVGCGVGNTVFPILQTNNDPGLFVYCCDFSSTAIELVQTNSEYDPSRCFAFVHDLCDEEKSYPVPKGSLDIIILIFVLSAIVPDKMQKAINRLSRLLKPGGMVLLRDYGRYDMAQLRFKKGQCLSGNFYVRGDGTRVYFFTQEELDTLFTTAGLEKVQNLVDRRLQVNRGKQLTMYRVWIQCKYCKPLLSSTS Predict reactive species Full Name: methyltransferase like 2B Calculated Molecular Weight: 313 aa, 36 kDa Observed Molecular Weight: 36~43 kDa GenBank Accession Number: BC107586 Gene Symbol: METTL2B Gene ID (NCBI): 55798 RRID: AB_3671804 Conjugate: Unconjugated Form: Liquid Purification Method: Protein A purification UNIPROT ID: Q6P1Q9 Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20 o C storage. 20ul sizes contain 0.1% BSA.