Proteintech, 84259-5-RR, CD117/c-Kit Recombinant monoclonal antibody

Size: 20ul / 100ul The CD117/c-Kit (84259-5-RR) by Proteintech is a Recombinant antibody targeting CD117/c-Kit in WB, IHC, IF/ICC, ELISA applications with reactivity to human samples 84259-5-RR targets CD117/c-Kit in WB, IHC, IF/ICC, ELISA applications and shows reactivity with human samples. Tested Applications Positive WB detected in: Mo7e cells Positive IHC detected in: human colon tissueNote: suggested antigen retrieval withTE buffer pH 9.0;(*) Alternatively, antigen retrieval may be performed withcitrate buffer pH 6.0 Positive IF/ICC detected in: Mo7e cells Recommended dilution Western Blot (WB): WB : 1:5000-1:50000 Immunohistochemistry (IHC): IHC : 1:250-1:1000 Immunofluorescence (IF)/ICC: IF/ICC : 1:200-1:800 Background Information CD117, also known as c-Kit and SCFR, is a transmembrane protein with tyrosine kinase activity encoded by the oncogene c-kit (PMID: 2448137). It is a member of the type III receptor tyrosine kinase family, which also includes CSF-1R, PDGFRβ, PDGFRα, and FLT3 (PMID: 29518044). CD117 is expressed on hematopoietic stem cells and progenitor cells, mast cells, and is also found in a wide range of non-haemopoietic cell types (including melanocytes, germ cells, astrocytes, renal tubules, breast glandular epithelial cells, sweat glands, and interstitial cells of Cajal) (PMID: 10582338; 23073628). CD117 plays an important role in early hemopoiesis. It is also involved in pigmentation, fertility, gut movement, and some aspects of the nervous system (PMID: 23073628). Specification Tested Reactivity: human Host / Isotype: Rabbit / IgG Class: Recombinant Type: Antibody Immunogen: Recombinant Protein Predict reactive species Full Name: v-kit Hardy-Zuckerman 4 feline sarcoma viral oncogene homolog Calculated Molecular Weight: 976 aa, 110 kDa Observed Molecular Weight: 140 kDa GenBank Accession Number: BC071593 Gene Symbol: CD117 Gene ID (NCBI): 3815 RRID: AB_3671807 Conjugate: Unconjugated Form: Liquid Purification Method: Protein A purfication UNIPROT ID: P10721 Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20 o C storage. 20ul sizes contain 0.1% BSA.