Proteintech, 84323-1-RR, RC3H1 Recombinant monoclonal antibody

Size: 20ul / 100ul The RC3H1 (84323-1-RR) by Proteintech is a Recombinant antibody targeting RC3H1 in WB, ELISA applications with reactivity to human samples 84323-1-RR targets RC3H1 in WB, ELISA applications and shows reactivity with human samples. Tested Applications Positive WB detected in: HepG2 cells, HeLa cells, HEK-293T cells, SH-SY5Y cells, MCF-7 cells Recommended dilution Western Blot (WB): WB : 1:1000-1:6000 Background Information Roquin-1 (Rc3h1) is an E3 ubiquitin ligase originally discovered in a mutational screen for genetic factors contributory to systemic lupus erythematosus-like symptoms in mice. A single base pair mutation in the Rc3h1 gene resulted in the manifestation of autoantibody production and sustained immunological inflammation characterized by excessive T follicular helper cell activation and formation of germinal centers. (PMID: 26673963) Specification Tested Reactivity: human Host / Isotype: Rabbit / IgG Class: Recombinant Type: Antibody Immunogen: Peptide Predict reactive species Full Name: ring finger and CCCH-type zinc finger domains 1 Observed Molecular Weight: 130-140 kDa GenBank Accession Number: NM_172071 Gene Symbol: RC3H1 Gene ID (NCBI): 149041 RRID: AB_3671865 Conjugate: Unconjugated Form: Liquid Purification Method: Protein A purfication UNIPROT ID: Q5TC82 Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20 o C storage. 20ul sizes contain 0.1% BSA.