Proteintech, 84428-3-RR, IL-10RA Recombinant monoclonal antibody

Size: 20ul / 100ul The IL-10RA (84428-3-RR) by Proteintech is a Recombinant antibody targeting IL-10RA in WB, ELISA applications with reactivity to human samples 84428-3-RR targets IL-10RA in WB, ELISA applications and shows reactivity with human samples. Tested Applications Positive WB detected in: HepG2 cells, K-562 cells Recommended dilution Western Blot (WB): WB : 1:200-1:1000 Background Information Interleukin-10 (IL-10) is an anti-inflammatory cytokine that plays a critical role in maintaining immune system balance. IL-10 signals through a receptor complex consisting of two molecules of the IL-10R alpha-chain (IL-10RA) and two molecules of the accessory IL-10R beta-chain (IL-10RB) (PMID: 22236434). IL-10RA and IL-10RB are members of the type II cytokine receptor family. IL-10RA, also known as IL-10R1 or CD210, is expressed on most hematopoietic cells at a basal level but is upregulated by various cells upon activation (PMID: 24507158). IL-10RA serves as the ligand-binding subunit of the receptor complex. Upon binding of IL-10 to the IL-10R complex, the kinases JAK1 and TYK2 are activated and catalyze the phosphorylation of IL-10RA at specific intracellular tyrosine residues, leading to the recruitment and subsequent phosphorylation of STAT3 (PMID: 11244051; 10433356). Specification Tested Reactivity: human Host / Isotype: Rabbit / IgG Class: Recombinant Type: Antibody Immunogen: FusionProtein Predict reactive species Full Name: interleukin 10 receptor, alpha Calculated Molecular Weight: 578 aa, 63 kDa Observed Molecular Weight: 90 kDa GenBank Accession Number: BC028082 Gene Symbol: IL-10RA Gene ID (NCBI): 3587 RRID: AB_3671955 Conjugate: Unconjugated Form: Liquid Purification Method: Protein A purfication UNIPROT ID: Q13651 Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20 o C storage. 20ul sizes contain 0.1% BSA.