Proteintech, 84528-1-RR, ALCAM Recombinant monoclonal antibody

Size: 20ul / 100ul The ALCAM (84528-1-RR) by Proteintech is a Recombinant antibody targeting ALCAM in WB, IHC, ELISA applications with reactivity to mouse samples 84528-1-RR targets ALCAM in WB, IHC, ELISA applications and shows reactivity with mouse samples. Tested Applications Positive WB detected in: mouse lung tissue Positive IHC detected in: mouse liver tissueNote: suggested antigen retrieval withTE buffer pH 9.0;(*) Alternatively, antigen retrieval may be performed withcitrate buffer pH 6.0 Recommended dilution Western Blot (WB): WB : 1:2000-1:10000 Immunohistochemistry (IHC): IHC : 1:250-1:1000 Background Information Activated leukocyte cell adhesion molecule (ALCAM, also known as CD166) is a cell adhesion molecule that belongs to the immunoglobulin superfamily. It is involved in cell-cell adhesion through homophilic and heterophilic (to CD6) interactions. ALCAM is widely expressed in a variety of normal tissues. Altered ALCAM expression has been associated with the differentiation state and progression in some neoplasms including melanoma, prostate, colorectal, and breast cancers (PMID: 20461761; 18172759). Specification Tested Reactivity: mouse Host / Isotype: Rabbit / IgG Class: Recombinant Type: Antibody Immunogen: Recombinant protein Predict reactive species Full Name: activated leukocyte cell adhesion molecule Calculated Molecular Weight: 65 kDa Observed Molecular Weight: 90-100 kDa GenBank Accession Number: NM_009655.3 Gene Symbol: Alcam Gene ID (NCBI): 11658 RRID: AB_3672036 Conjugate: Unconjugated Form: Liquid Purification Method: Protein A purification UNIPROT ID: Q61490 Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20 o C storage. 20ul sizes contain 0.1% BSA.