Proteintech, 84691-1-RR, Phospho-MEK1 (Ser298) Recombinant monoclonal antibody

Size: 20ul / 100ul The Phospho-MEK1 (Ser298) (84691-1-RR) by Proteintech is a Recombinant antibody targeting Phospho-MEK1 (Ser298) in WB, FC (Intra), ELISA applications with reactivity to human samples 84691-1-RR targets Phospho-MEK1 (Ser298) in WB, FC (Intra), ELISA applications and shows reactivity with human samples. Tested Applications Positive WB detected in: Calyculin A treated PC-3 cells Positive FC (Intra) detected in: Calyculin A treated HEK-293 cells Recommended dilution Western Blot (WB): WB : 1:5000-1:50000 Flow Cytometry (FC) (INTRA): FC (INTRA) : 0.13 ug per 10^6 cells in a 100 µl suspension Background Information MAP2K1 encodes MAPK1, also known as MEK1. MEK1 variants can enhance MEK1 expression and ERK1 phosphorylation that together lead to continuous activation of MEK/ERK signaling pathway. MEK1 bind directly to ERK2 through a region in the N terminus of MEK. In addition, a proline-rich (PR) regulatory sequence in MEK is also involved in MEK-ERK association and signal propagation. The coupling between MEK1 and ERK2 is enhanced through phosphorylation on S298 in the MEK1 PR region, whereas phosphorylation on MEK1 T292 releases the complex. MEK1 enzymatic activity is regulated by site-specific phosphorylation that can be activated with phosphorylation of Ser217/Ser221 by Raf kinase or suppressed by phosphorylation of Thr286 and Thr292 by CDK1 and CDK5 or Thr292 and Thr386 by ERK1/2. (PMID: 31972311, PMID: 17928366, PMID: 22177953) Specification Tested Reactivity: human Cited Reactivity: human Host / Isotype: Rabbit / IgG Class: Recombinant Type: Antibody Immunogen: Peptide Predict reactive species Full Name: mitogen-activated protein kinase kinase 1 Calculated Molecular Weight: 43 kDa Observed Molecular Weight: 40-45 kDa GenBank Accession Number: BC139729 Gene Symbol: MEK1 Gene ID (NCBI): 5604 ENSEMBL Gene ID: ENSG00000169032 RRID: AB_3672107 Conjugate: Unconjugated Form: Liquid Purification Method: Protein A purfication UNIPROT ID: Q02750 Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20 o C storage. 20ul sizes contain 0.1% BSA.