Proteintech, 84794-1-RR, gp130/IL6ST Recombinant monoclonal antibody

Size: 20ul / 100ul The gp130/IL6ST (84794-1-RR) by Proteintech is a Recombinant antibody targeting gp130/IL6ST in IHC, ELISA applications with reactivity to mouse samples 84794-1-RR targets gp130/IL6ST in IHC, ELISA applications and shows reactivity with mouse samples. Tested Applications Positive IHC detected in: mouse spleen tissueNote: suggested antigen retrieval withTE buffer pH 9.0;(*) Alternatively, antigen retrieval may be performed withcitrate buffer pH 6.0 Recommended dilution Immunohistochemistry (IHC): IHC : 1:200-1:800 Background Information GP130, also known as IL6ST, is a transmembrane protein that serves as a shared signal-transducing subunit for a variety of cytokines(PMID: 39414364), including interleukin-6 (IL-6), IL-11, IL-27, leukemia inhibitory factor (LIF), ciliary neurotrophic factor (CNTF), oncostatin M (OSM), cardiotrophin-1 (CT-1), cardiotrophin-like cytokine (CLC)/CLC factor 1 (CLCF-1), and neuropoietin (PMID: 24099136). This protein is highly conserved among vertebrates and plays a crucial role in mediating interactions between a wide range of immune and non-immune cells. GP130 was originally discovered in 1978 and was successfully cloned and expressed in 1990, leading to a deeper understanding of cytokine signaling pathways (PMID: 308233). Specification Tested Reactivity: mouse Host / Isotype: Rabbit / IgG Class: Recombinant Type: Antibody Immunogen: CatNo: Eg1631 Product name: Recombinant Mouse gp130/IL6ST protein (rFc Tag)(HPLC verified) Source: mammalian cells -derived, pHZ-KIsec-C-rFc Tag: C-rFc Domain: 23-617 aa of NM_010560.3 Sequence: QLLEPCGYIYPEFPVVQRGSNFTAICVLKEACLQHYYVNASYIVWKTNHAAVPREQVTVINRTTSSVTFTDVVLPSVQLTCNILSFGQIEQNVYGVTMLSGFPPDKPTNLTCIVNEGKNMLCQWDPGRETYLETNYTLKSEWATEKFPDCQSKHGTSCMVSYMPTYYVNIEVWVEAENALGKVSSESINFDPVDKVKPTPPYNLSVTNSEELSSILKLSWVSSGLGGLLDLKSDIQYRTKDASTWIQVPLEDTMSPRTSFTVQDLKPFTEYVFRIRSIKDSGKGYWSDWSEEASGTTYEDRPSRPPSFWYKTNPSHGQEYRSVRLIWKALPLSEANGKILDYEVILTQSKSVSQTYTVTGTELTVNLTNDRYVASLAARNKVGKSAAAVLTIPSPHVTAAYSVVNLKAFPKDNLLWVEWTPPPKPVSKYILEWCVLSENAPCVEDWQQEDATVNRTHLRGRLLESKCYQITVTPVFATGPGGSESLKAYLKQAAPARGPTVRTKKVGKNEAVLAWDQIPVDDQNGFIRNYSISYRTSVGKEMVVHVDSSHTEYTLSSLSSDTLYMVRMAAYTDEGGKDGPEFTFTTPKFAQGEIE Predict reactive species Full Name: interleukin 6 signal transducer Calculated Molecular Weight: 102kDa, 917aa GenBank Accession Number: NM_010560.3 Gene Symbol: Il6st Gene ID (NCBI): 16195 Conjugate: Unconjugated Form: Liquid Purification Method: Protein A purification UNIPROT ID: Q00560 Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20 o C storage. 20ul sizes contain 0.1% BSA.