Proteintech, 84808-5-RR, Arginase-2 Recombinant monoclonal antibody

Size: 20ul / 100ul The Arginase-2 (84808-5-RR) by Proteintech is a Recombinant antibody targeting Arginase-2 in WB, IHC, ELISA applications with reactivity to human, mouse samples 84808-5-RR targets Arginase-2 in WB, IHC, ELISA applications and shows reactivity with human, mouse samples. Tested Applications Positive WB detected in: U-251 cells, mouse kidney tissue, K-562 cells, Caco-2 cells Positive IHC detected in: mouse kidney tissueNote: suggested antigen retrieval withTE buffer pH 9.0;(*) Alternatively, antigen retrieval may be performed withcitrate buffer pH 6.0 Recommended dilution Western Blot (WB): WB : 1:5000-1:50000 Immunohistochemistry (IHC): IHC : 1:1000-1:4000 Background Information Arginase 2 is composed of 354 amino acid residues, including an NH2-terminal presequence for mitochondrial targeting and import. In the mitochondria, ornithine generated by Arginase 2 will give rise to glutamate via ornithine aminotransferase (OAT). Glutamate participates in several transamination reactions, including forming α-ketoglutarate (αKG) that may enter the TCA cycle and increase cycle intermediates and flux.Arginase 1 is mainly expressed in hepatocytes, and mice with a disruption of Arginase 1 gene die soon after birth. Arginase 2 is poorly expressed in hepatocytes, and most highly expressed in kidney, prostate, and immune cells such as monocyte/ macrophages. (PMID: 25234945,PMID: 27214549) Specification Tested Reactivity: human, mouse Host / Isotype: Rabbit / IgG Class: Recombinant Type: Antibody Immunogen: CatNo: Ag6609 Product name: Recombinant human ARG2 protein Source: e coli. -derived, PET28a Tag: 6*His Domain: 1-354 aa of BC001350 Sequence: MSLRGSLSRLLQTRVHSILKKSVHSVAVIGAPFSQGQKRKGVEHGPAAIREAGLMKRLSSLGCHLKDFGDLSFTPVPKDDLYNNLIVNPRSVGLANQELAEVVSRAVSDGYSCVTLGGDHSLAIGTISGHARHCPDLCVVWVDAHADINTPLTTSSGNLHGQPVSFLLRELQDKVPQLPGFSWIKPCISSASIVYIGLRDVDPPEHFILKNYDIQYFSMRDIDRLGIQKVMERTFDLLIGKRQRPIHLSFDIDAFDPTLAPATGTPVVGGLTYREGMYIAEEIHNTGLLSALDLVEVNPQLATSEEEAKTTANLAVDVIASSFGQTREGGHIVYDQLPTPSSPDESENQARVRI Predict reactive species Full Name: arginase, type II Calculated Molecular Weight: 39 kDa Observed Molecular Weight: 39 kDa GenBank Accession Number: BC001350 Gene Symbol: ARG2 Gene ID (NCBI): 384 Conjugate: Unconjugated Form: Liquid Purification Method: Protein A purfication UNIPROT ID: P78540 Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20 o C storage. 20ul sizes contain 0.1% BSA.