Proteintech, 84834-1-RR, Phospho-POLR2A (Ser5) Recombinant monoclonal antibody

Size: 20ul / 100ul The Phospho-POLR2A (Ser5) (84834-1-RR) by Proteintech is a Recombinant antibody targeting Phospho-POLR2A (Ser5) in WB, IF/ICC, FC (Intra), ELISA, ChIP-qPCR applications with reactivity to human samples 84834-1-RR targets Phospho-POLR2A (Ser5) in WB, IF/ICC, FC (Intra), ELISA, ChIP-qPCR applications and shows reactivity with human samples. Tested Applications Positive WB detected in: HeLa cells, λ phosphatase treated HeLa cells Positive IF/ICC detected in: HeLa cells Positive FC (Intra) detected in: λ phosphatase treated HeLa cells Positive ChIP-qPCR detected in: HeLa cells Recommended dilution Western Blot (WB): WB : 1:5000-1:50000 Immunofluorescence (IF)/ICC: IF/ICC : 1:200-1:800 Flow Cytometry (FC) (INTRA): FC (INTRA) : 0.13 ug per 10^6 cells in a 100 µl suspension CHIP-QPCR: CHIP-QPCR : 1:10-1:100 Background Information RNA Pol II consists of 12 subunits and is responsible for the expression of mRNA and lincRNA genes within cells. The subunits of RNA polymerases have been shown to play specific roles in Pol II CTD dephosphorylation, tissue-specific transcription, mRNA decay, and translation. Pol II transcription involves initiation through recruitment and CTD serine 5 phosphorylation, elongation with CTD serine 2 phosphorylation, and termination. (PMID: 38842922) Specification Tested Reactivity: human Host / Isotype: Rabbit / IgG Class: Recombinant Type: Antibody Immunogen: Peptide Predict reactive species Full Name: polymerase (RNA) II (DNA directed) polypeptide A, 220kDa Calculated Molecular Weight: 1970 aa, 217 kDa Observed Molecular Weight: 270 kDa GenBank Accession Number: BC137231 Gene Symbol: POLR2A Gene ID (NCBI): 5430 RRID: AB_3672142 Conjugate: Unconjugated Form: Liquid Purification Method: Protein A purfication UNIPROT ID: P24928 Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20 o C storage. 20ul sizes contain 0.1% BSA.