Proteintech, 84846-4-RR, REST Recombinant monoclonal antibody

Size: 20ul / 100ul The REST (84846-4-RR) by Proteintech is a Recombinant antibody targeting REST in WB, IF/ICC, ELISA, ChIP-qPCR applications with reactivity to human samples 84846-4-RR targets REST in WB, IF/ICC, ELISA, ChIP-qPCR applications and shows reactivity with human samples. Tested Applications Positive WB detected in: HeLa cells, HepG2 cells, K-562 cells, BxPC-3 cells, SW 1990 cells, L02 cells Positive IF/ICC detected in: HeLa cells Positive ChIP-qPCR detected in: HeLa cells Recommended dilution Western Blot (WB): WB : 1:2000-1:10000 Immunofluorescence (IF)/ICC: IF/ICC : 1:200-1:800 CHIP-QPCR: CHIP-QPCR : 1:10-1:100 Background Information REST also named as NRSF or X2 box repressor is a 1097 amino acid protein, which contains 9 C2H2-type zinc fingers. REST as a transcriptional repressor binds neuron-restrictive silencer element (NRSE) and represses neuronal gene transcription in non-neuronal cells. Repressor element 1 (RE1) silencing transcription factor (REST), is widely expressed during embryogenesis and plays a strategic role in terminal neuronal differentiation. The molecular weight of REST is 121 kDa, but REST was detected at 200 kDa by western blot in our result, which is similar to the article (PMID: 16764822). Specification Tested Reactivity: human Host / Isotype: Rabbit / IgG Class: Recombinant Type: Antibody Immunogen: CatNo: Ag17707 Product name: Recombinant human REST protein Source: e coli. -derived, PET28a Tag: 6*His Domain: 748-1097 aa of BC132859 Sequence: ELSPPMEVVQKEPVKIELSPPIEVVQKEPVQMELSPPMGVVQKEPAQREPPPPREPPLHMEPISKKPPLRKDKKEKSNMQSERARKEQVLIEVGLVPVKDSWLLKESVSTEDLSPPSPPLPKENLREEASGDQKLLNTGEGNKEAPLQKVGAEEADESLPGLAANINESTHISSSGQNLNTPEGETLNGKHQTDSIVCEMKMDTDQNTRENLTGINSTVEEPVSPMLPPSAVEEREAVSKTALASPPATMAANESQEIDEDEGIHSHEGSDLSDNMSEGSDDSGLHGARPVPQESSRKNAKEALAVKAAKGDFVCIFCDRSFRKGKDYSKHLNRHLVNVYYLEEAAQGQE Predict reactive species Full Name: RE1-silencing transcription factor Calculated Molecular Weight: 1097 aa, 122 kDa Observed Molecular Weight: 200 kDa GenBank Accession Number: BC132859 Gene Symbol: REST Gene ID (NCBI): 5978 Conjugate: Unconjugated Form: Liquid Purification Method: Protein A purfication UNIPROT ID: Q13127 Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20 o C storage. 20ul sizes contain 0.1% BSA.