Proteintech, 84879-5-RR, Methylmalonyl Coenzyme A mutase/MUT Recombinant monoclonal antibody

Size: 20ul / 100ul The Methylmalonyl Coenzyme A mutase/MUT (84879-5-RR) by Proteintech is a Recombinant antibody targeting Methylmalonyl Coenzyme A mutase/MUT in WB, IHC, FC (Intra), ELISA applications with reactivity to human, mouse, rat samples 84879-5-RR targets Methylmalonyl Coenzyme A mutase/MUT in WB, IHC, FC (Intra), ELISA applications and shows reactivity with human, mouse, rat samples. Tested Applications Positive WB detected in: HEK-293 cells, HeLa cells, HepG2 cells, K-562 cells, mouse brain tissue, rat liver tissue Positive IHC detected in: human prostate cancer tissueNote: suggested antigen retrieval withTE buffer pH 9.0;(*) Alternatively, antigen retrieval may be performed withcitrate buffer pH 6.0 Positive FC (Intra) detected in: A431 cells Recommended dilution Western Blot (WB): WB : 1:5000-1:50000 Immunohistochemistry (IHC): IHC : 1:200-1:800 Flow Cytometry (FC) (INTRA): FC (INTRA) : 0.25 ug per 10^6 cells in a 100 µl suspension Background Information Methylmalonyl Coenzyme A mutase (MUT) is an enzyme that plays a crucial role in the metabolism of certain amino acids and fatty acids. Mutations in the MUT gene can lead to methylmalonic acidemia, a metabolic disorder characterized by the accumulation of toxic compounds such as methylmalonyl-CoA and propionyl-CoA. This condition can cause severe health issues including developmental delays, metabolic acidosis, and neurological problems. MUT is essential for maintaining normal metabolic processes and its dysfunction can have significant health implications, highlighting its importance in both basic metabolism and clinical medicine. Specification Tested Reactivity: human, mouse, rat Host / Isotype: Rabbit / IgG Class: Recombinant Type: Antibody Immunogen: CatNo: Ag10523 Product name: Recombinant human MUT protein Source: e coli. -derived, PGEX-4T Tag: GST Domain: 401-750 aa of BC016282 Sequence: SARIARNTQIIIQEESGIPKVADPWGGSYMMECLTNDVYDAALKLINEIEEMGGMAKAVAEGIPKLRIEECAARRQARIDSGSEVIVGVNKYQLEKEDTVEVLAIDNTSVRNRQIEKLKKIKSSRDQALAERCLAALTECAASGDGNILALAVDASRARCTVGEITDALKKVFGEHKANDRMVSGAYRQEFGESKEITSAIKRVHKFMEREGRRPRLLVAKMGQDGHDRGAKVIATGFADLGFDVDIGPLFQTPREVAQQAVDADVHAVGVSTLAAGHKTLVPELIKELNSLGRPDILVMCGGVIPPQDYEFLFEVGVSNVFGPGTRIPKAAVQVLDDIEKCLEKKQQSV Predict reactive species Full Name: methylmalonyl Coenzyme A mutase Calculated Molecular Weight: 750 aa, 83 kDa Observed Molecular Weight: 78 kDa GenBank Accession Number: BC016282 Gene Symbol: MUT Gene ID (NCBI): 4594 Conjugate: Unconjugated Form: Liquid Purification Method: Protein A purfication UNIPROT ID: P22033 Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20 o C storage. 20ul sizes contain 0.1% BSA.