Proteintech, 84916-5-RR, FEN1 Recombinant monoclonal antibody

Size: 20ul / 100ul The FEN1 (84916-5-RR) by Proteintech is a Recombinant antibody targeting FEN1 in WB, IHC, IF/ICC, FC (Intra), ELISA applications with reactivity to human, mouse samples 84916-5-RR targets FEN1 in WB, IHC, IF/ICC, FC (Intra), ELISA applications and shows reactivity with human, mouse samples. Tested Applications Positive WB detected in: A431 cells, Jurkat cells, HeLa cells, U-937 cells, Y79 cells, NIH/3T3 cells, mouse testis tissue Positive IHC detected in: human colon cancer tissueNote: suggested antigen retrieval withTE buffer pH 9.0;(*) Alternatively, antigen retrieval may be performed withcitrate buffer pH 6.0 Positive IF/ICC detected in: HeLa cells Positive FC (Intra) detected in: HeLa cells Recommended dilution Western Blot (WB): WB : 1:5000-1:50000 Immunohistochemistry (IHC): IHC : 1:500-1:2000 Immunofluorescence (IF)/ICC: IF/ICC : 1:250-1:1000 Flow Cytometry (FC) (INTRA): FC (INTRA) : 0.25 ug per 10^6 cells in a 100 µl suspension Background Information FEN1(Flap endonuclease-1) is the prototypical member of the 5'-nuclease superfamily, whose activities span a range of cellular pathways involved in DNA replication and genome maintenance (PMID: 22118811, 21496641, 20929870). FEN1 is a structure-selective metallonuclease essential for Okazaki fragment maturation through efficient removal of 5' flaps resulting from strand displacement during lagging-strand synthesis (PMID: 8144677, 9081985). FEN1 is overexpressed in multiple cancer types, and has been suggested both as a biomarker relating to prognosis and disease progression and as a potential therapeutic target (PMID: 19010819, 16879693, 19596913, 27526030). Specification Tested Reactivity: human, mouse Host / Isotype: Rabbit / IgG Class: Recombinant Type: Antibody Immunogen: CatNo: Ag6552 Product name: Recombinant human FEN1 protein Source: e coli. -derived, PGEX-4T Tag: GST Domain: 1-380 aa of BC000323 Sequence: MGIQGLAKLIADVAPSAIRENDIKSYFGRKVAIDASMSIYQFLIAVRQGGDVLQNEEGETTSHLMGMFYRTIRMMENGIKPVYVFDGKPPQLKSGELAKRSERRAEAEKQLQQAQAAGAEQEVEKFTKRLVKVTKQHNDECKHLLSLMGIPYLDAPSEAEASCAALVKAGKVYAAATEDMDCLTFGSPVLMRHLTASEAKKLPIQEFHLSRILQELGLNQEQFVDLCILLGSDYCESIRGIGPKRAVDLIQKHKSIEEIVRRLDPNKYPVPENWLHKEAHQLFLEPEVLDPESVELKWSEPNEEELIKFMCGEKQFSEERIRSGVKRLSKSRQGSTQGRLDDFFKVTGSLSSAKRKEPEPKGSTKKKAKTGAAGKFKRGK Predict reactive species Full Name: flap structure-specific endonuclease 1 Calculated Molecular Weight: 43 kDa Observed Molecular Weight: 48 kDa GenBank Accession Number: BC000323 Gene Symbol: FEN1 Gene ID (NCBI): 2237 Conjugate: Unconjugated Form: Liquid Purification Method: Protein A purification UNIPROT ID: P39748 Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20 o C storage. 20ul sizes contain 0.1% BSA.