Proteintech, 84954-4-RR, CA2 Recombinant monoclonal antibody

Size: 20ul / 100ul The CA2 (84954-4-RR) by Proteintech is a Recombinant antibody targeting CA2 in WB, ELISA applications with reactivity to human, mouse, rat samples 84954-4-RR targets CA2 in WB, ELISA applications and shows reactivity with human, mouse, rat samples. Tested Applications Positive WB detected in: A431 cells, HEK-293 cells, mouse kidney tissue, rat kidney tissue, mouse ovary tissue Recommended dilution Western Blot (WB): WB : 1:5000-1:50000 Background Information CA2(carbonic anhydrase II ) is also called Car2, CA-II, and CAII and belongs to the alpha-carbonic anhydrase family. It reversibly hydrates CO2 in cellular ion transport and homeostasis. CA2 is essential for bone resorption and osteoclast differentiation. It regulates SLC9A1 activity via a phosphorylation-regulated CAII binding site in SLC9A1 tail(PMID:16475831). It also may be involved in brain development (PMID:16825953). Defects in CA2 cause osteopetrosis autosomal recessive type 3 (OPTB3). Specification Tested Reactivity: human, mouse, rat Host / Isotype: Rabbit / IgG Class: Recombinant Type: Antibody Immunogen: CatNo: Ag10680 Product name: Recombinant human CA2 protein Source: e coli. -derived, PGEX-4T Tag: GST Domain: 1-260 aa of BC011949 Sequence: MSHHWGYGKHNGPEHWHKDFPIAKGERQSPVDIDTHTAKYDPSLKPLSVSYDQATSLRILNNGHAFNVEFDDSQDKAVLKGGPLDGTYRLIQFHFHWGSLDGQGSEHTVDKKKYAAELHLVHWNTKYGDFGKAVQQPDGLAVLGIFLKVGSAKPGLQKVVDVLDSIKTKGKSADFTNFAARGLLPESLDYWTYPGSLTTPPLLECVTWIVLKEPISVSSEQVLKFRKLNFNGEGEPEELMVDNWRPAQPLKNRQIKASFK Predict reactive species Full Name: carbonic anhydrase II Calculated Molecular Weight: 260 aa, 29 kDa Observed Molecular Weight: 29 kDa GenBank Accession Number: BC011949 Gene Symbol: CA2 Gene ID (NCBI): 760 Conjugate: Unconjugated Form: Liquid Purification Method: Protein A purification UNIPROT ID: P00918 Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20 o C storage. 20ul sizes contain 0.1% BSA.