Proteintech, 84974-1-RR, Phospho-POLR2A (Ser2) Recombinant monoclonal antibody

Size: 20ul / 100ul The Phospho-POLR2A (Ser2) (84974-1-RR) by Proteintech is a Recombinant antibody targeting Phospho-POLR2A (Ser2) in WB, FC (Intra), ELISA, ChIP-qPCR applications with reactivity to human samples 84974-1-RR targets Phospho-POLR2A (Ser2) in WB, FC (Intra), ELISA, ChIP-qPCR applications and shows reactivity with human samples. Tested Applications Positive WB detected in: HEK-293 cells, λ phosphatase treated HEK-293 cells Positive FC (Intra) detected in: λ phosphatase treated HEK-293 cells Positive ChIP-qPCR detected in: HeLa cells Recommended dilution Western Blot (WB): WB : 1:5000-1:50000 Flow Cytometry (FC) (INTRA): FC (INTRA) : 0.06 ug per 10^6 cells in a 100 µl suspension CHIP-QPCR: CHIP-QPCR : 1:10-1:100 Background Information DNA-directed RNA polymerase II subunit RPB1 is the catalytic core component of DNA-dependent RNA polymerase II (Pol II). So that the information encoded in DNA can be used, RNA polymerase II makes copies of specific genes. To ensure that RNA polymerase II copies the correct genes at the correct time, a group of regulatory proteins are needed to control its activity. Many of these proteins interact with RNA polymerase II at a region known as the C-terminal domain, or CTD for short. For example, before RNA polymerase can make a full copy of a gene, a small molecule called a phosphate group must first be added to CTD at specific units known as Ser2. (PMID: 31385803) Specification Tested Reactivity: human Host / Isotype: Rabbit / IgG Class: Recombinant Type: Antibody Immunogen: Peptide Predict reactive species Full Name: polymerase (RNA) II (DNA directed) polypeptide A, 220kDa Calculated Molecular Weight: 1970 aa, 217 kDa Observed Molecular Weight: 250 kDa GenBank Accession Number: BC137231 Gene Symbol: POLR2A Gene ID (NCBI): 5430 Conjugate: Unconjugated Form: Liquid Purification Method: Protein A purfication UNIPROT ID: P24928 Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20 o C storage. 20ul sizes contain 0.1% BSA.