Proteintech, 84981-1-RR, Phospho-EIF4E (Ser209) Recombinant monoclonal antibody

Size: 20ul / 100ul The Phospho-EIF4E (Ser209) (84981-1-RR) by Proteintech is a Recombinant antibody targeting Phospho-EIF4E (Ser209) in WB, FC (Intra), ELISA applications with reactivity to human samples 84981-1-RR targets Phospho-EIF4E (Ser209) in WB, FC (Intra), ELISA applications and shows reactivity with human samples. Tested Applications Positive WB detected in: Calyculin A treated HEK-293T cells Positive FC (Intra) detected in: Calyculin A treated HEK-293T cells Recommended dilution Western Blot (WB): WB : 1:2000-1:10000 Flow Cytometry (FC) (INTRA): FC (INTRA) : 0.13 ug per 10^6 cells in a 100 µl suspension Background Information Eukaryotic initiation factor 4F (eIF4F) is a critical effector and is thought to be the rate-limiting determinant of translation. The activity of eIF4E can be regulated by phosphorylation at Ser209, which is controlled by mitogen-activated protein kinase-interacting kinases Mnk1 and Mnk2. Although the function of eIF4E phosphorylation remains the subject of debate, it has been documented that the phosphorylation of eIF4E at Ser209 is crucial for biogenesis, tumorigenesis, and viral infection. (PMID: 38299843) Specification Tested Reactivity: human Host / Isotype: Rabbit / IgG Class: Recombinant Type: Antibody Immunogen: Peptide Predict reactive species Full Name: eukaryotic translation initiation factor 4E Calculated Molecular Weight: 29 kDa Observed Molecular Weight: 25 kDa GenBank Accession Number: BC012611 Gene Symbol: EIF4E Gene ID (NCBI): 1977 Conjugate: Unconjugated Form: Liquid Purification Method: Protein A purfication UNIPROT ID: P06730 Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20 o C storage. 20ul sizes contain 0.1% BSA.