Product Description
Size: 20ul / 100ul
The RPS4X (85040-5-RR) by Proteintech is a Recombinant antibody targeting RPS4X in WB, IF/ICC, FC (Intra), ELISA applications with reactivity to human, mouse, rat samples
85040-5-RR targets RPS4X in WB, IF/ICC, FC (Intra), ELISA applications and shows reactivity with human, mouse, rat samples.
Tested Applications
Positive WB detected in: HT-29 cells, HepG2 cells, HeLa cells, MCF-7 cells, mouse spleen tissue, mouse brain tissue, rat liver tissue
Positive IF/ICC detected in: MCF-7 cells
Positive FC (Intra) detected in: HeLa cells
Recommended dilution
Western Blot (WB): WB : 1:5000-1:50000
Immunofluorescence (IF)/ICC: IF/ICC : 1:250-1:1000
Flow Cytometry (FC) (INTRA): FC (INTRA) : 0.25 ug per 10^6 cells in a 100 µl suspension
Background Information
RPS4X, a X-linked gene in the human, encodes a subunit of ribosomal protein S4, a component of the 40S subunit and 60S subunit. Ribosomal protein S4 is the only ribosomal protein known to be encoded by more than one gene, namely this gene, RPS4Y2 and the ribosomal protein S4, X-linked (RPS4X). (PMID:8358435)
Specification
Tested Reactivity: human, mouse, rat
Host / Isotype: Rabbit / IgG
Class: Recombinant
Type: Antibody
Immunogen: CatNo: Ag6513 Product name: Recombinant human SCAR protein Source: e coli. -derived, PGEX-4T Tag: GST Domain: 1-263 aa of BC000472 Sequence: MARGPKKHLKRVAAPKHWMLDKLTGVFAPRPSTGPHKLRECLPLIIFLRNRLKYALTGDEVKKICMQRFIKIDGKVRTDITYPAGFMDVISIDKTGENFRLIYDTKGRFAVHRITPEEAKYKLCKVRKIFVGTKGIPHLVTHDARTIRYPDPLIKVNDTIQIDLETGKITDFIKFDTGNLCMVTGGANLGRIGVITNRERHPGSFDVVHVKDANGNSFATRLSNIFVIGKGNKPWISLPRGKGIRLTIAEERDKRLAAKQSSG Predict reactive species
Full Name: ribosomal protein S4, X-linked
Calculated Molecular Weight: 263 aa, 30 kDa
Observed Molecular Weight: 28-30 kDa
GenBank Accession Number: BC000472
Gene Symbol: RPS4X
Gene ID (NCBI): 6191
Conjugate: Unconjugated
Form: Liquid
Purification Method: Protein A purification
UNIPROT ID: P62701
Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20 o C storage. 20ul sizes contain 0.1% BSA.
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Collaboration
Tony Tang
Email: Tony.Tang@iright.com
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