Proteintech, 85044-1-RR, Phospho-PAK1 (Ser144)/PAK2 (Ser141) Recombinant monoclonal antibody

Size: 20ul / 100ul The Phospho-PAK1 (Ser144)/PAK2 (Ser141) (85044-1-RR) by Proteintech is a Recombinant antibody targeting Phospho-PAK1 (Ser144)/PAK2 (Ser141) in WB, ELISA applications with reactivity to human, mouse, rat samples 85044-1-RR targets Phospho-PAK1 (Ser144)/PAK2 (Ser141) in WB, ELISA applications and shows reactivity with human, mouse, rat samples. Tested Applications Positive WB detected in: pervanadate treated C6 cells, LPS treated RAW 264.7 cells Recommended dilution Western Blot (WB): WB : 1:1000-1:4000 Background Information The human PAK family is divided into the group I (PAK1 to PAK3) and group II (PAK4 to PAK6). Group I PAK share some domains that are not present in the group II members. In particular, the autoinhibitory domain (AID) is important for regulation of the kinase activity of the group I family members. Autophosphorylation at PAK1 Ser144, or at the equivalent sites for the other PAK, stabilizes the open conformation and sustains high kinase activity. Mutation of tyrosines 131 or 429 is associated with reduced dimerization and enhanced kinase activity. (PMID: 31748572) Specification Tested Reactivity: human, mouse, rat Cited Reactivity: mouse Host / Isotype: Rabbit / IgG Class: Recombinant Type: Antibody Immunogen: Peptide Predict reactive species Full Name: p21 protein (Cdc42/Rac)-activated kinase 1 Calculated Molecular Weight: 553 aa, 62 kDa Observed Molecular Weight: 61 kDa GenBank Accession Number: BC109299 Gene Symbol: PAK1 Gene ID (NCBI): 5058 Conjugate: Unconjugated Form: Liquid Purification Method: Protein A purfication UNIPROT ID: Q13153 Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20 o C storage. 20ul sizes contain 0.1% BSA.