Proteintech, 85097-1-RR, VLDLR Recombinant monoclonal antibody

Size: 20ul / 100ul The VLDLR (85097-1-RR) by Proteintech is a Recombinant antibody targeting VLDLR in WB, FC (Intra), ELISA applications with reactivity to human samples 85097-1-RR targets VLDLR in WB, FC (Intra), ELISA applications and shows reactivity with human samples. Tested Applications Positive WB detected in: MCF-7 cells, THP-1 cells Positive FC (Intra) detected in: THP-1 cells Recommended dilution Western Blot (WB): WB : 1:5000-1:50000 Flow Cytometry (FC) (INTRA): FC (INTRA) : 0.25 ug per 10^6 cells in a 100 µl suspension Background Information The very low-density lipoprotein receptor (VLDLR) is a member of the LDL receptor family with a complex molecular weight profile due to post-translational modifications. The calculated molecular weight of the core protein is approximately 84-86 kDa, however, VLDLR undergoes extensive glycosylation during processing, resulting in higher apparent molecular weights observed in SDS-PAGE analyses. Multiple studies report that glycosylated VLDLR migrates as broad bands between 90-180 kDa under reducing conditions, with the most commonly observed forms at approximately 96 kDa (core glycosylated form), 115-135 kDa (mature fully glycosylated form), and 150-180 kDa (hyperglycosylated or dimeric forms). Specification Tested Reactivity: human Host / Isotype: Rabbit / IgG Class: Recombinant Type: Antibody Immunogen: Peptide Predict reactive species Full Name: very low density lipoprotein receptor Calculated Molecular Weight: 96 kDa Observed Molecular Weight: 100-120 kDa GenBank Accession Number: NM_003383 Gene Symbol: VLDLR Gene ID (NCBI): 7436 Conjugate: Unconjugated Form: Liquid Purification Method: Protein A purfication UNIPROT ID: P98155 Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20 o C storage. 20ul sizes contain 0.1% BSA.