Proteintech, 85119-2-RR, AKAP1 Recombinant monoclonal antibody

Size: 20ul / 100ul The AKAP1 (85119-2-RR) by Proteintech is a Recombinant antibody targeting AKAP1 in WB, IHC, IF/ICC, FC (Intra), ELISA applications with reactivity to human samples 85119-2-RR targets AKAP1 in WB, IHC, IF/ICC, FC (Intra), ELISA applications and shows reactivity with human samples. Tested Applications Positive WB detected in: HEK-293 cells, HeLa cells, HepG2 cells, MCF-7 cells, PC-3 cells, Caco-2 cells Positive IHC detected in: human colon cancer tissueNote: suggested antigen retrieval withTE buffer pH 9.0;(*) Alternatively, antigen retrieval may be performed withcitrate buffer pH 6.0 Positive IF/ICC detected in: HepG2 cells, MCF-7 cells Positive FC (Intra) detected in: HEK-293T cells Recommended dilution Western Blot (WB): WB : 1:5000-1:50000 Immunohistochemistry (IHC): IHC : 1:50-1:500 Immunofluorescence (IF)/ICC: IF/ICC : 1:250-1:1000 Flow Cytometry (FC) (INTRA): FC (INTRA) : 0.13 ug per 10^6 cells in a 100 µl suspension Background Information AKAP1 (also termed AKAP149) is a human 149 kDa anchoring protein localized in mitochondria and the endoplasmic reticulum/nuclear envelope (ER-NE) network. It Binds to type I and II regulatory subunits of protein kinase A and anchors them to the cytoplasmic face of the mitochondrial outer membrane. This protein is speculated to be involved in the cAMP-dependent signal transduction pathway and in directing RNA to a specific cellular compartment. Specification Tested Reactivity: human Host / Isotype: Rabbit / IgG Class: Recombinant Type: Antibody Immunogen: CatNo: Ag8037 Product name: Recombinant human AKAP1 protein Source: e coli. -derived, PET28a Tag: 6*His Domain: 31-351 aa of BC000729 Sequence: GHVSSHDEQQVEAGAVQLRADPAIKEPLPVEDVCPKVVSTPPSVTEPPEKELSTVSKLPAEPPALLQTHPPCRRSESSGILPNTTDMRLRPGTRRDDSTKLELALTGGEAKSIPLECPLSSPKGVLFSSKSAEVCKQDSPFSRVPRKVQPGYPVVPAEKRSSGERARETGGAEGTGDAVLGEKVLEEALLSREHVLELENSKGPSLASLEGEEDKGKSSSSQVVGPVQEEEYVAEKLPSRFIESAHTELAKDDAAPAPPVADAKAQDRGVEGELGNEESLDRNEEGLDRNEEGLDRNEESLDRNEEGLDRNEEIKRAAFQI Predict reactive species Full Name: A kinase (PRKA) anchor protein 1 Calculated Molecular Weight: 97 kDa Observed Molecular Weight: 149 kDa GenBank Accession Number: BC000729 Gene Symbol: AKAP1 Gene ID (NCBI): 8165 Conjugate: Unconjugated Form: Liquid Purification Method: Protein A purification UNIPROT ID: Q92667 Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20 o C storage. 20ul sizes contain 0.1% BSA.