Proteintech, 85161-4-RR, NUP214 Recombinant monoclonal antibody

Size: 20ul / 100ul The NUP214 (85161-4-RR) by Proteintech is a Recombinant antibody targeting NUP214 in WB, IHC, IF/ICC, ELISA applications with reactivity to human, mouse samples 85161-4-RR targets NUP214 in WB, IHC, IF/ICC, ELISA applications and shows reactivity with human, mouse samples. Tested Applications Positive WB detected in: K-562 cells, HeLa cells, HEK-293T cells, MCF-7 cells, A431 cells, NIH/3T3 cells Positive IHC detected in: mouse kidney tissue, mouse testis tissueNote: suggested antigen retrieval withTE buffer pH 9.0;(*) Alternatively, antigen retrieval may be performed withcitrate buffer pH 6.0 Positive IF/ICC detected in: K-562 cells Recommended dilution Western Blot (WB): WB : 1:500-1:2000 Immunohistochemistry (IHC): IHC : 1:50-1:500 Immunofluorescence (IF)/ICC: IF/ICC : 1:250-1:1000 Background Information NUP214 is also known as CAN and is an FG nucleoporin anchored to the cytoplasmic ring of the nuclear pore complex (NPC) and forms a subcomplex with nucleoporin NUP88. NUP214 is a component of the NPC with a key role in protein and mRNA nuclear export. NUP214 is a target for chromosomal translocations involved in leukemogenesis (PMID: 17264208, 30669574). NUP214 is expressed in thymus, spleen, bone marrow, kidney, brain, and testis, but hardly in all other tissues or in whole embryos during development. The observed molecular weight of NUP214 is 250 kDa, which is consistent with the description in the literature (PMID: 12191473). Specification Tested Reactivity: human, mouse Host / Isotype: Rabbit / IgG Class: Recombinant Type: Antibody Immunogen: CatNo: Ag21377 Product name: Recombinant human NUP214 protein Source: e coli. -derived, PET30a Tag: 6*His Domain: 1-350 aa of BC105998 Sequence: MGDEMDAMIPEREMKDFQFRALKKVRIFDSPEELPKERSSLLAVSNKYGLVFAGGASGLQIFPTKNLLIQNKPGDDPNKIVDKVQGLLVPMKFPIHHLALSCDNLTLSACMMSSEYGSIIAFFDVRTFSNEAKQQKRPFAYHKLLKDAGGMVIDMKWNPTVPSMVAVCLADGSIAVLQVTETVKVCATLPSTVAVTSVCWSPKGKQLAVGKQNGTVVQYLPTLQEKKVIPCPPFYESDHPVRVLDVLWIGTYVFAIVYAAADGTLETSPDVVMALLPKKEEKHPEIFVNFMEPCYGSCTERQHHYYLSYIEEWDLVLAASAASTEVSILARQSDQINWESWLLEDSSRAE Predict reactive species Full Name: nucleoporin 214kDa Calculated Molecular Weight: 2080 aa, 213 kDa Observed Molecular Weight: 250 kDa GenBank Accession Number: BC105998 Gene Symbol: NUP214 Gene ID (NCBI): 8021 Conjugate: Unconjugated Form: Liquid Purification Method: Protein A purification UNIPROT ID: P35658 Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20 o C storage. 20ul sizes contain 0.1% BSA.