Proteintech, 85223-3-RR, Phospho-PDPK1 (Ser241) Recombinant monoclonal antibody

Size: 20ul / 100ul The Phospho-PDPK1 (Ser241) (85223-3-RR) by Proteintech is a Recombinant antibody targeting Phospho-PDPK1 (Ser241) in WB, IF/ICC, FC (Intra), ELISA applications with reactivity to human, mouse samples 85223-3-RR targets Phospho-PDPK1 (Ser241) in WB, IF/ICC, FC (Intra), ELISA applications and shows reactivity with human, mouse samples. Tested Applications Positive WB detected in: NIH/3T3 cells Positive IF/ICC detected in: NIH/3T3 cells Positive FC (Intra) detected in: NIH/3T3 cells Recommended dilution Western Blot (WB): WB : 1:1000-1:6000 Immunofluorescence (IF)/ICC: IF/ICC : 1:50-1:500 Flow Cytometry (FC) (INTRA): FC (INTRA) : 0.13 ug per 10^6 cells in a 100 µl suspension Background Information 3-Phosphoinositide dependent protein kinase-1 (PDK1 or PDPK1) is a serine-threonine kinase belonging to AGC kinase family. PDK1 plays a critical role in establishing ACD (Asymmetric cell division) in the epithelium (PMID: 27184845). The kinase activity of PDK1 depends on phosphatidyl inositol 3-kinase (PI3K), a key intermediate in signaling pathways including those from growth factor receptors and adhesion molecules. Substrates of PDK1, including AKT and the protein kinase C (PKC) isozymes, regulate a number of essential cell functions (PMID: 20027184). PDPK1 has 5 isoforms produced by alternative splicing of 48~63 kDa and is detected as 60-63 kDa. Specification Tested Reactivity: human, mouse Host / Isotype: Rabbit / IgG Class: Recombinant Type: Antibody Immunogen: Peptide Predict reactive species Full Name: 3-phosphoinositide dependent protein kinase-1 Calculated Molecular Weight: 556 aa, 63 kDa Observed Molecular Weight: 58-68 kDa GenBank Accession Number: BC012103 Gene Symbol: PDPK1 Gene ID (NCBI): 5170 Conjugate: Unconjugated Form: Liquid Purification Method: Protein A purification UNIPROT ID: O15530 Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20 o C storage. 20ul sizes contain 0.1% BSA.