Proteintech, 85288-2-RR, ATP7B-Specific Recombinant monoclonal antibody

Size: 20ul / 100ul The ATP7B-Specific (85288-2-RR) by Proteintech is a Recombinant antibody targeting ATP7B-Specific in WB, IHC, IF-P, FC (Intra), ELISA applications with reactivity to human, mouse, rat samples 85288-2-RR targets ATP7B-Specific in WB, IHC, IF-P, FC (Intra), ELISA applications and shows reactivity with human, mouse, rat samples. Tested Applications Positive WB detected in: mouse liver tissue, rat liver tissue Positive IHC detected in: mouse liver tissueNote: suggested antigen retrieval withTE buffer pH 9.0;(*) Alternatively, antigen retrieval may be performed withcitrate buffer pH 6.0 Positive IF-P detected in: mouse liver tissue Positive FC (Intra) detected in: HepG2 cells Recommended dilution Western Blot (WB): WB : 1:5000-1:50000 Immunohistochemistry (IHC): IHC : 1:1000-1:4000 Immunofluorescence (IF)-P: IF-P : 1:200-1:800 Flow Cytometry (FC) (INTRA): FC (INTRA) : 0.25 ug per 10^6 cells in a 100 µl suspension Background Information ATP7B, also named as PWD, WC1 and WND, belongs to the cation transport ATPase (P-type) family and Type IB subfamily. ATP7B is involved in the export of copper out of the cells, such as the efflux of hepatic copper into the bile. ATP7B catalyzes the reaction: ATP + H2O + Cu2+(In) = ADP + phosphate + Cu2+(Out). Defects in ATP7B are the cause of Wilson disease (WD). This antibody is specific to ATP7B. Specification Tested Reactivity: human, mouse, rat Host / Isotype: Rabbit / IgG Class: Recombinant Type: Antibody Immunogen: Peptide Predict reactive species Full Name: ATPase, Cu++ transporting, beta polypeptide Calculated Molecular Weight: 157 kDa Observed Molecular Weight: 150 kDa GenBank Accession Number: NM_000053 Gene Symbol: ATP7B Gene ID (NCBI): 540 Conjugate: Unconjugated Form: Liquid Purification Method: Protein A purification UNIPROT ID: P35670 Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20 o C storage. 20ul sizes contain 0.1% BSA.