Proteintech, 85327-1-RR, MRPL3 Recombinant monoclonal antibody

Size: 20ul / 100ul The MRPL3 (85327-1-RR) by Proteintech is a Recombinant antibody targeting MRPL3 in WB, IHC, ELISA applications with reactivity to human samples 85327-1-RR targets MRPL3 in WB, IHC, ELISA applications and shows reactivity with human samples. Tested Applications Positive WB detected in: HepG2 cells, A431 cells, HeLa cells, HEK-293T cells Positive IHC detected in: human liver cancer tissueNote: suggested antigen retrieval withTE buffer pH 9.0;(*) Alternatively, antigen retrieval may be performed withcitrate buffer pH 6.0 Recommended dilution Western Blot (WB): WB : 1:5000-1:50000 Immunohistochemistry (IHC): IHC : 1:200-1:800 Background Information MRPL3, also named as 39S ribosomal protein L3, mitochondrial or RPML3, is a 348 amino acid protein, which belongs to the ribosomal protein L3P family. MRPL3 localizes in the Mitochondrion and has an association with combined oxidative phosphorylation deficiency 9. MRPL3 plays role in the mitochondrial proteins biosynthesis and ribosomal structure and biogenesis. Specification Tested Reactivity: human Host / Isotype: Rabbit / IgG Class: Recombinant Type: Antibody Immunogen: CatNo: Ag9970 Product name: Recombinant human MRPL3 protein Source: e coli. -derived, PGEX-4T Tag: GST Domain: 1-348 aa of BC003375 Sequence: MPGWRLLTQVGAQVLGRLGDGLGAALGPGNRTHIWLFVRGLHGKSGTWWDEHLSEENVPFIKQLVSDEDKAQLASKLCPLKDEPWPIHPWEPGSFRVGLIALKLGMMPLWTKDGQKHVVTLLQVQDCHVLKYTSKENCNGKMATLSVGGKTVSRFRKATSILEFYRELGLPPKQTVKIFNITDNAAIKPGTPLYAAHFRPGQYVDVTAKTIGKGFQGVMKRWGFKGQPATHGQTKTHRRPGAVATGDIGRVWPGTKMPGKMGNIYRTEYGLKVWRINTKHNIIYVNGSVPGHKNCLVKVKDSKLPAYKDLGKNLPFPTYFPDGDEEELPEDLYDENVCQPGAPSITFA Predict reactive species Full Name: mitochondrial ribosomal protein L3 Calculated Molecular Weight: 39 kDa Observed Molecular Weight: 35-39 kDa GenBank Accession Number: BC003375 Gene Symbol: MRPL3 Gene ID (NCBI): 11222 Conjugate: Unconjugated Form: Liquid Purification Method: Protein A purification UNIPROT ID: P09001 Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20 o C storage. 20ul sizes contain 0.1% BSA.