Proteintech, 85400-4-RR, VAMP8 Recombinant monoclonal antibody

Size: 20ul / 100ul The VAMP8 (85400-4-RR) by Proteintech is a Recombinant antibody targeting VAMP8 in WB, IHC, IF/ICC, ELISA applications with reactivity to human, mouse, rat samples 85400-4-RR targets VAMP8 in WB, IHC, IF/ICC, ELISA applications and shows reactivity with human, mouse, rat samples. Tested Applications Positive WB detected in: THP-1 cells, HL-60 cells, A431 cells, HeLa cells, rat kidney tissue, mouse kidney tissue Positive IHC detected in: human placenta tissueNote: suggested antigen retrieval withTE buffer pH 9.0;(*) Alternatively, antigen retrieval may be performed withcitrate buffer pH 6.0 Positive IF/ICC detected in: THP-1 cells Recommended dilution Western Blot (WB): WB : 1:5000-1:50000 Immunohistochemistry (IHC): IHC : 1:1000-1:4000 Immunofluorescence (IF)/ICC: IF/ICC : 1:200-1:800 Background Information VAMP8, also named as endobrevin, is a member of the vesicle-associated membrane protein (VAMP)/synaptobrevin family and the SNARE (soluble NSF-attachment protein receptor) superfamily. Characterized by a common sequence called the SNARE motif, SNARE proteins are involved in membrane fusion and vesicular transport (PMID: 11252968). VAMP8 is involved in autophagy through the direct control of autophagosome membrane fusion with the lysososome membrane. It is required for dense-granule secretion in platelets and plays a role in regulated enzyme secretion in pancreatic acinar cells. VAMP8 is also involved in the abscission of the midbody during cell division, which leads to completely separate daughter cells. Specification Tested Reactivity: human, mouse, rat Host / Isotype: Rabbit / IgG Class: Recombinant Type: Antibody Immunogen: CatNo: Ag7903 Product name: Recombinant human VAMP8 protein Source: e coli. -derived, PGEX-4T Tag: GST Domain: 1-81 aa of BC001634 Sequence: MEEASEGGGNDRVRNLQSEVEGVKNIMTQNVERILARGENLEHLRNKTEDLEATSEHFKTTSQKVARKFWWKNVKMIVLIC Predict reactive species Full Name: vesicle-associated membrane protein 8 (endobrevin) Calculated Molecular Weight: 11 kDa Observed Molecular Weight: 15 kDa GenBank Accession Number: BC001634 Gene Symbol: VAMP8 Gene ID (NCBI): 8673 Conjugate: Unconjugated Form: Liquid Purification Method: Protein A purification UNIPROT ID: Q9BV40 Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20 o C storage. 20ul sizes contain 0.1% BSA.