Proteintech, 85698-1-RR, SCN4A Recombinant monoclonal antibody

Size: 20ul / 100ul The SCN4A (85698-1-RR) by Proteintech is a Recombinant antibody targeting SCN4A in WB, IHC, ELISA applications with reactivity to human, mouse samples 85698-1-RR targets SCN4A in WB, IHC, ELISA applications and shows reactivity with human, mouse samples. Tested Applications Positive WB detected in: K-562 cells, MCF-7 cells, COLO 205 cells Positive IHC detected in: mouse skeletal muscle tissueNote: suggested antigen retrieval withTE buffer pH 9.0;(*) Alternatively, antigen retrieval may be performed withcitrate buffer pH 6.0 Recommended dilution Western Blot (WB): WB : 1:5000-1:50000 Immunohistochemistry (IHC): IHC : 1:400-1:1600 Background Information Sodium channel protein type 4 subunit alpha (SCN4A, also known as Nav1.4) is the pore-forming subunit of the primary sodium channel present in skeletal muscles (PMID: 16193245). Nav1.4 related channelopathies that affect skeletal muscle excitability are dominant diseases classified in two opposite groups as defined by the prevalent clinical symptoms: muscle stiffness and hypertonia (myotonia) episodes [non dystrophic myotonias (NDM)], and muscle weakness resulting in paralysis episodes (periodic paralyses; PP) (PMID: 20237798; 26285000). Specification Tested Reactivity: human, mouse Host / Isotype: Rabbit / IgG Class: Recombinant Type: Antibody Immunogen: Peptide Predict reactive species Full Name: sodium channel, voltage-gated, type IV, alpha subunit Calculated Molecular Weight: 208 kDa Observed Molecular Weight: 208 kDa GenBank Accession Number: NM_000334 Gene Symbol: SCN4A Gene ID (NCBI): 6329 Conjugate: Unconjugated Form: Liquid Purification Method: Protein A purification UNIPROT ID: P35499 Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20 o C storage. 20ul sizes contain 0.1% BSA.