Proteintech, 85712-1-RR, SPATA2 Recombinant monoclonal antibody

Size: 20ul / 100ul The SPATA2 (85712-1-RR) by Proteintech is a Recombinant antibody targeting SPATA2 in WB, IHC, FC (Intra), ELISA applications with reactivity to human, mouse samples 85712-1-RR targets SPATA2 in WB, IHC, FC (Intra), ELISA applications and shows reactivity with human, mouse samples. Tested Applications Positive WB detected in: HeLa cells, MCF-7 cells, Raji cells, PC-3 cells, A549 cells, mouse testis tissue Positive IHC detected in: mouse testis tissueNote: suggested antigen retrieval withTE buffer pH 9.0;(*) Alternatively, antigen retrieval may be performed withcitrate buffer pH 6.0 Positive FC (Intra) detected in: MCF-7 cells Recommended dilution Western Blot (WB): WB : 1:5000-1:50000 Immunohistochemistry (IHC): IHC : 1:50-1:500 Flow Cytometry (FC) (INTRA): FC (INTRA) : 0.25 ug per 10^6 cells in a 100 µl suspension Specification Tested Reactivity: human, mouse Host / Isotype: Rabbit / IgG Class: Recombinant Type: Antibody Immunogen: CatNo: Ag30773 Product name: Recombinant human SPATA2 protein Source: e coli. -derived, PET28a Tag: 6*His Domain: 330-520 aa of BC009481 Sequence: STQDDVDLYTDSEPRATYRRQDALRPDVWLLRNDAHSLYHKRSPPAKESALSKCQSCGLSCSSSLCQRCDSLLTCPPASKPSAFPSKASTHDSLAHGASLREKYPGQTQGLDRLPHLHSKSKPSTTPTSRCGFCNRPGATNTCTQCSKVSCDACLSAYHYDPCYKKSELHKFMPNNQLNYKSTQLSHLVYR Predict reactive species Full Name: spermatogenesis associated 2 Calculated Molecular Weight: 58 kDa Observed Molecular Weight: 58-60 kDa GenBank Accession Number: BC009481 Gene Symbol: SPATA2 Gene ID (NCBI): 9825 Conjugate: Unconjugated Form: Liquid Purification Method: Protein A purification UNIPROT ID: Q9UM82 Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20 o C storage. 20ul sizes contain 0.1% BSA.