Proteintech, 85753-1-RR, Phospho-IRAK4 (Thr345/Ser346) Recombinant monoclonal antibody

Size: 20ul / 100ul The Phospho-IRAK4 (Thr345/Ser346) (85753-1-RR) by Proteintech is a Recombinant antibody targeting Phospho-IRAK4 (Thr345/Ser346) in WB, IHC, ELISA applications with reactivity to human, rat samples 85753-1-RR targets Phospho-IRAK4 (Thr345/Ser346) in WB, IHC, ELISA applications and shows reactivity with human, rat samples. Tested Applications Positive WB detected in: IL-1 beta treated Karpas-299 cells Positive IHC detected in: rat lymph node tissueNote: suggested antigen retrieval withTE buffer pH 9.0;(*) Alternatively, antigen retrieval may be performed withcitrate buffer pH 6.0 Recommended dilution Western Blot (WB): WB : 1:5000-1:50000 Immunohistochemistry (IHC): IHC : 1:1000-1:4000 Background Information IRAK4 is the central kinase in the Toll-like receptor pathway of the innate immune system. Toll-like receptors constitute the first line of defense against pathogenic micro-organisms such as bacteria, viruses, and yeast. Unphosphorylated full-length IRAK4 can autophosphorylate in vitro in a cell-free system. In the cellular context, however, autophosphorylation is inducible by IL-1β. Four phosphorylation sites have been identified in the activation loop in some articles, which are the results of autophosphorylation: Thr-342, Thr-345, Ser-346 and Thr-352. (PMID: 24567333) Specification Tested Reactivity: human, rat Host / Isotype: Rabbit / IgG Class: Recombinant Type: Antibody Immunogen: Peptide Predict reactive species Full Name: interleukin-1 receptor-associated kinase 4 Calculated Molecular Weight: 52 kDa Observed Molecular Weight: 55 kDa GenBank Accession Number: BC013316 Gene Symbol: IRAK4 Gene ID (NCBI): 51135 Conjugate: Unconjugated Form: Liquid Purification Method: Protein A purification UNIPROT ID: Q9NWZ3 Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20 o C storage. 20ul sizes contain 0.1% BSA.