Proteintech, 86033-1-RR, MPRIP Recombinant monoclonal antibody

Size: 20ul / 100ul The MPRIP (86033-1-RR) by Proteintech is a Recombinant antibody targeting MPRIP in WB, IF-P, ELISA applications with reactivity to human samples 86033-1-RR targets MPRIP in WB, IF-P, ELISA applications and shows reactivity with human samples. Tested Applications Positive WB detected in: HeLa cells, A549 cells, SKOV-3 cells Positive IF-P detected in: mouse brain tissue Recommended dilution Western Blot (WB): WB : 1:1000-1:6000 Immunofluorescence (IF)-P: IF-P : 1:200-1:800 Background Information MPRIP, also named as KIAA0864, MRIP, RHOIP3 and p116Rip, is required for the regulation of the actin cytoskeleton by RhoA and ROCK1. MRPIP targets myosin phosphatase to the actin cytoskeleton. Depletion leads to an increased number of stress fibers in smooth muscle cells through stabilization of actin fibers by phosphorylated myosin. Overexpression of MRIP as well as its F-actin-binding region leads to disassembly of stress fibers in neuronal cells. The antibody recognizes the C-term of isoform2 and isoform3 of MPRIP. Specification Tested Reactivity: human Host / Isotype: Rabbit / IgG Class: Recombinant Type: Antibody Immunogen: Peptide Predict reactive species Full Name: myosin phosphatase Rho interacting protein Calculated Molecular Weight: 117 kDa Observed Molecular Weight: 140 kDa GenBank Accession Number: NM_015134 Gene Symbol: MPRIP Gene ID (NCBI): 23164 Conjugate: Unconjugated Form: Liquid Purification Method: Protein A purification UNIPROT ID: Q6WCQ1 Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20 o C storage. 20ul sizes contain 0.1% BSA.