Proteintech, 86052-1-RR, Napsin A Recombinant monoclonal antibody

Size: 20ul / 100ul The Napsin A (86052-1-RR) by Proteintech is a Recombinant antibody targeting Napsin A in WB, ELISA applications with reactivity to human samples 86052-1-RR targets Napsin A in WB, ELISA applications and shows reactivity with human samples. Tested Applications Positive WB detected in: A549 cells, K-562 cells, human kidney tissue Recommended dilution Western Blot (WB): WB : 1:1000-1:6000 Background Information Napsin is found in two isoforms, napsin A and B, with highly homologous nucleotide sequences (91.2%). Napsin A appears to be a functional proteinase, predominantly expressed in lung and kidney. Napsin B is transcribed exclusively in cells related to the immune system and lacks an in-frame stop codon and is believed to be a pseudogene.(PMID:12698189). Napsin A is superior to TTF-1 in distinguishing primary lung ACA from other carcinomas (except kidney), particularly primary lung small cell carcinoma, and primary thyroid carcinoma.(PMID:22288963). Specification Tested Reactivity: human Host / Isotype: Rabbit / IgG Class: Recombinant Type: Antibody Immunogen: CatNo: Ag9721 Product name: Recombinant human NAPSA protein Source: e coli. -derived, PET28a Tag: 6*His Domain: 1-420 aa of BC017842 Sequence: MSPPPLLQPLLLLLPLLNVEPSGATLIRIPLHRVQPGRRTLNLLRGWREPAELPKLGAPSPGDKPIFVPLSNYRDVQYFGEIGLGTPPQNFTVAFDTGSSNLWVPSRRCHFFSVPCWLHHRFDPKASSSFQANGTKFAIQYGTGRVDGILSEDKLTIGGIKGASVIFGEALWEPSLVFAFAHFDGILGLGFPILSVEGVRPPMDVLVEQGLLDKPVFSFYLNRDPEEPDGGELVLGGSDPAHYIPPLTFVPVTVPAYWQIHMERVKVGPGLTLCAKGCAAILDTGTSLITGPTEEIRALHAAIGGIPLLAGEYIILCSEIPKLPAVSFLLGGVWFNLTAHDYVIQTTRNGVRLCLSGFQALDVPPPAGPFWILGDVFLGTYVAVFDRGDMKSSARVGLARARTRGADLGWGETAQAQFPG Predict reactive species Full Name: napsin A aspartic peptidase Calculated Molecular Weight: 420 aa, 45 kDa Observed Molecular Weight: 40-45 kDa GenBank Accession Number: BC017842 Gene Symbol: Napsin A Gene ID (NCBI): 9476 Conjugate: Unconjugated Form: Liquid Purification Method: Protein A purification UNIPROT ID: O96009 Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20 o C storage. 20ul sizes contain 0.1% BSA.