Proteintech, 86073-2-RR, MAG/Siglec-4a Recombinant monoclonal antibody

Size: 20ul / 100ul The MAG/Siglec-4a (86073-2-RR) by Proteintech is a Recombinant antibody targeting MAG/Siglec-4a in WB, IHC, ELISA applications with reactivity to mouse, rat samples 86073-2-RR targets MAG/Siglec-4a in WB, IHC, ELISA applications and shows reactivity with mouse, rat samples. Tested Applications Positive WB detected in: rat brain tissue, unboiled rat brain tissue, rat cerebellum tissue, mouse brain tissue, rat spinal cord tissue Positive IHC detected in: rat brain tissueNote: suggested antigen retrieval withTE buffer pH 9.0;(*) Alternatively, antigen retrieval may be performed withcitrate buffer pH 6.0 Recommended dilution Western Blot (WB): WB : 1:5000-1:50000 Immunohistochemistry (IHC): IHC : 1:500-1:2000 Specification Tested Reactivity: mouse, rat Host / Isotype: Rabbit / IgG Class: Recombinant Type: Antibody Immunogen: CatNo: Eg3251 Product name: Recombinant Rat MAG protein (rFc Tag) Source: mammalian cells -derived, pHZ-KIsec-C-rFc Tag: C-rFc Domain: 20-516 aa of NM_017190.4 Sequence: GHWGAWMPSSISAFEGTCVSIPCRFDFPDELRPAVVHGVWYFNSPYPKNYPPVVFKSRTQVVHESFQGRSRLLGDLGLRNCTLLLSTLSPELGGKYYFRGDLGGYNQYTFSEHSVLDIINTPNIVVPPEVVAGTEVEVSCMVPDNCPELRPELSWLGHEGLGEPTVLGRLREDEGTWVQVSLLHFVPTREANGHRLGCQAAFPNTTLQFEGYASLDVKYPPVIVEMNSSVEAIEGSHVSLLCGADSNPPPLLTWMRDGMVLREAVAESLYLDLEEVTPAEDGIYACLAENAYGQDNRTVELSVMYAPWKPTVNGTVVAVEGETVSILCSTQSNPDPILTIFKEKQILATVIYESQLQLELPAVTPEDDGEYWCVAENQYGQRATAFNLSVEFAPIILLESHCAAARDTVQCLCVVKSNPEPSVAFELPSRNVTVNETEREFVYSERSGLLLTSILTLRGQAQAPPRVICTSRNLYGTQSLELPFQGAHRLMWAKIGP Predict reactive species Full Name: myelin-associated glycoprotein Calculated Molecular Weight: 69 kDa Observed Molecular Weight: 70-100 kDa GenBank Accession Number: NM_017190.4 Gene Symbol: Mag Gene ID (NCBI): 29409 Conjugate: Unconjugated Form: Liquid Purification Method: Protein A purification UNIPROT ID: P07722-1 Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20 o C storage. 20ul sizes contain 0.1% BSA.