Proteintech, 86165-3-RR, GABARAP Recombinant monoclonal antibody

Size: 20ul / 100ul The GABARAP (86165-3-RR) by Proteintech is a Recombinant antibody targeting GABARAP in WB, IF/ICC, ELISA applications with reactivity to human, mouse, rat samples 86165-3-RR targets GABARAP in WB, IF/ICC, ELISA applications and shows reactivity with human, mouse, rat samples. Tested Applications Positive WB detected in: HEK-293 cells, HeLa cells, SH-SY5Y cells, RAW 264.7cells, NIH/3T3 cells, mouse brain tissue, rat brain tissue Positive IF/ICC detected in: Chloroquine treated HepG2 cells Recommended dilution Western Blot (WB): WB : 1:5000-1:50000 Immunofluorescence (IF)/ICC: IF/ICC : 1:250-1:1000 Background Information GABARAP (Gamma-aminobutyric acid type A receptor-associated protein), was initially identified as GABAA receptor binding protein and has shown to be implicated in intracellular trafficking of GABAA receptors. Through interacting with a broad rang of proteins, GABARAP has been demonstrated as a multifunctional protein implicated in intracellular vesicle trafficking, apoptosis, and autophagy. The protein level of lipidated form of GABARAP (GABARAP-II) is upregulated during the autophagic process, which makes it as an autophagic marker. This antibody detected endogenous GABARAP protein in lysates from various tissues. It may cross-react with GABARAPL1 due to the high identity of amino acid sequence. Specification Tested Reactivity: human, mouse, rat Host / Isotype: Rabbit / IgG Class: Recombinant Type: Antibody Immunogen: Peptide Predict reactive species Full Name: GABA(A) receptor-associated protein Calculated Molecular Weight: 14 kDa Observed Molecular Weight: 14-18 kDa GenBank Accession Number: BC106748 Gene Symbol: GABARAP Gene ID (NCBI): 11337 Conjugate: Unconjugated Form: Liquid Purification Method: Protein A purification UNIPROT ID: O95166 Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20 o C storage. 20ul sizes contain 0.1% BSA.