Proteintech, 86170-2-RR, SF3A1 Recombinant monoclonal antibody

Size: 20ul / 100ul The SF3A1 (86170-2-RR) by Proteintech is a Recombinant antibody targeting SF3A1 in WB, IF/ICC, ELISA applications with reactivity to human, rat samples 86170-2-RR targets SF3A1 in WB, IF/ICC, ELISA applications and shows reactivity with human, rat samples. Tested Applications Positive WB detected in: HeLa cells, rat kidney tissue, HEK-293 cells, K-562 cells, HL-60 cells, LNCaP cells, Neuro-2a cells Positive IF/ICC detected in: HeLa cells Recommended dilution Western Blot (WB): WB : 1:2000-1:10000 Immunofluorescence (IF)/ICC: IF/ICC : 1:250-1:1000 Background Information SF3A1, also known as SF3a120, is a 793 amino acid protein, which is a Subunit of the splicing factor SF3A required for 'A' complex assembly formed by the stable binding of U2 snRNP to the branchpoint sequence (BPS) in pre-mRNA. SF3A1 also be involved in the assembly of the 'E' complex. The calculated molecular weight of SF3A1 is 88 kDa, but the modified SF3A1 is about 115-120 kDa.(PMID: 7489498 ) Specification Tested Reactivity: human, rat Host / Isotype: Rabbit / IgG Class: Recombinant Type: Antibody Immunogen: CatNo: Ag8196 Product name: Recombinant human SF3A1 protein Source: e coli. -derived, PET28a Tag: 6*His Domain: 443-793 aa of BC007684 Sequence: DRSIREKQSDDEVYAPGLDIESSLKQLAERRTDIFGVEETAIGKKIGEEEIQKPEEKVTWDGHSGSMARTQQAAQANITLQEQIEAIHKAKGLVPEDDTKEKIGPSKPNEIPQQPPPPSSATNIPSSAPPITSVPRPPTMPPPVRTTVVSAVPVMPRPPMASVVRLPPGSVIAPMPPIIHAPRINVVPMPPSAPPIMAPRPPPMIVPTAFVPAPPVAPVPAPAPMPPVHPPPPMEDEPTSKKLKTEDSLMPEEEFLRRNKGPVSIKVQVPNMQDKTEWKLNGQVLVFTLPLTDQVSVIKVKIHEATGMPAGKQKLQYEGIFIKDSNSLAYYNMANGAVIHLALKERGGRKK Predict reactive species Full Name: splicing factor 3a, subunit 1, 120kDa Calculated Molecular Weight: 793 aa, 89 kDa Observed Molecular Weight: 120 kDa GenBank Accession Number: BC007684 Gene Symbol: SF3A1 Gene ID (NCBI): 10291 Conjugate: Unconjugated Form: Liquid Purification Method: Protein A purification UNIPROT ID: Q15459 Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20 o C storage. 20ul sizes contain 0.1% BSA.