Proteintech, 86236-1-RR, FOXO4 Recombinant monoclonal antibody

Size: 20ul / 100ul The FOXO4 (86236-1-RR) by Proteintech is a Recombinant antibody targeting FOXO4 in WB, IHC, IF/ICC, ELISA applications with reactivity to human, mouse, rat samples 86236-1-RR targets FOXO4 in WB, IHC, IF/ICC, ELISA applications and shows reactivity with human, mouse, rat samples. Tested Applications Positive WB detected in: HCT 116 cells, HEK-293T cells, HuT 78 cells, Ramos cells, Jurkat cells, PC-12 cells, mouse heart tissue Positive IHC detected in: human heart tissueNote: suggested antigen retrieval withTE buffer pH 9.0;(*) Alternatively, antigen retrieval may be performed withcitrate buffer pH 6.0 Positive IF/ICC detected in: HEK-293T cells Recommended dilution Western Blot (WB): WB : 1:1000-1:8000 Immunohistochemistry (IHC): IHC : 1:500-1:2000 Immunofluorescence (IF)/ICC: IF/ICC : 1:50-1:500 Background Information FOXO4, also named as AFX, AFX1 and MLLT7, is a transcription factor that is involved in the regulation of the insulin signaling pathway. It binds to insulin-response elements (IREs) and can activate transcription of IGFBP1.FOXO downregulates the expression of HIF1A and suppresses hypoxia-induced transcriptional activation of HIF1A-modulated genes. It is involved in negative regulation of the cell cycle. A chromosomal aberration involving FOXO4 is found in acute leukemias. Specification Tested Reactivity: human, mouse, rat Host / Isotype: Rabbit / IgG Class: Recombinant Type: Antibody Immunogen: CatNo: Ag16169 Product name: Recombinant human FOXO4 protein Source: e coli. -derived, PGEX-4T Tag: GST Domain: 198-505 aa of BC106761 Sequence: MDSSSKLLRGRSKAPKKKPSVLPAPPEGATPTSPVGHFAKWSGSPCSRNREEADMWTTFRPRSSSNASSVSTRLSPLRPESEVLAEEIPASVSSYAGGVPPTLNEGLELLDGLNLTSSHSLLSRSGLSGFSLQHPGVTGPLHTYSSSLFSPAEGPLSAGEGCFSSSQALEALLTSDTPPPPADVLMTQVDPILSQAPTLLLLGGLPSSSKLATGVGLCPKPLEAPGPSSLVPTLSMIAPPPVMASAPIPKALGTPVLTPPTEAASQDRMPQDLDLDMYMENLECDMDNIISDLMDEGEGLDFNFEPDP Predict reactive species Full Name: forkhead box O4 Calculated Molecular Weight: 505 aa, 54 kDa Observed Molecular Weight: 65 kDa GenBank Accession Number: BC106761 Gene Symbol: FOXO4 Gene ID (NCBI): 4303 Conjugate: Unconjugated Form: Liquid Purification Method: Protein A purification UNIPROT ID: P98177 Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20 o C storage. 20ul sizes contain 0.1% BSA.