Proteintech, 86324-1-RR, Cytokeratin 10 Recombinant monoclonal antibody

Size: 20ul / 100ul The Cytokeratin 10 (86324-1-RR) by Proteintech is a Recombinant antibody targeting Cytokeratin 10 in WB, IHC, IF-P, ELISA applications with reactivity to human, mouse, rat samples 86324-1-RR targets Cytokeratin 10 in WB, IHC, IF-P, ELISA applications and shows reactivity with human, mouse, rat samples. Tested Applications Positive WB detected in: mouse skin tissue, rat skin tissue Positive IHC detected in: human skin tissue, human skin cancer tissueNote: suggested antigen retrieval withTE buffer pH 9.0;(*) Alternatively, antigen retrieval may be performed withcitrate buffer pH 6.0 Positive IF-P detected in: mouse skin tissue Recommended dilution Western Blot (WB): WB : 1:5000-1:50000 Immunohistochemistry (IHC): IHC : 1:2000-1:8000 Immunofluorescence (IF)-P: IF-P : 1:50-1:500 Background Information Cytokeratin 10 (CK-10, K10, KRT10) is a type I (acidic) cytokeratin and part of the intermediate filament protein superfamily. It is expressed in keratinized stratified epithelium, suprabasal cell layers (including the stratum corneum), and differentiated areas of highly differentiated squamous cell carcinomas. CK-10 plays a role in maintaining the structural integrity of epithelial cells and is involved in differentiation, tissue specialization, and epidermal barrier establishment. Mutations in the KRT10 gene are associated with epidermolytic hyperkeratosis, and its expression is linked to the terminal differentiation of epidermal cells . CK-10 is also used as a marker for tissue differentiation and characterization of malignant tumors in surgical pathology. Specification Tested Reactivity: human, mouse, rat Host / Isotype: Rabbit / IgG Class: Recombinant Type: Antibody Immunogen: Peptide Predict reactive species Full Name: keratin 10 Calculated Molecular Weight: 584 aa, 59 kDa Observed Molecular Weight: 59 kDa GenBank Accession Number: BC034697 Gene Symbol: Cytokeratin 10 Gene ID (NCBI): 3858 Conjugate: Unconjugated Form: Liquid Purification Method: Protein A purification UNIPROT ID: P13645 Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20 o C storage. 20ul sizes contain 0.1% BSA.