Proteintech, 86376-1-RR, G-CSF Recombinant monoclonal antibody

Size: 20ul / 100ul The G-CSF (86376-1-RR) by Proteintech is a Recombinant antibody targeting G-CSF in WB, IF/ICC, FC (Intra), ELISA applications with reactivity to human samples 86376-1-RR targets G-CSF in WB, IF/ICC, FC (Intra), ELISA applications and shows reactivity with human samples. Tested Applications Positive WB detected in: HeLa cells, HepG2 cells, K-562 cells, MCF-7 cells, MOLT-4 cells, A549 cells Positive IF/ICC detected in: K-562 cells Positive FC (Intra) detected in: K-562 cells Recommended dilution Western Blot (WB): WB : 1:1000-1:4000 Immunofluorescence (IF)/ICC: IF/ICC : 1:50-1:500 Flow Cytometry (FC) (INTRA): FC (INTRA) : 0.25 ug per 10^6 cells in a 100 µl suspension Background Information Granulocyte colony-stimulating factor (G-CSF), also referred to as CSF3, is a protective cytokine with anti-inflammatory effects. G-CSF is important in promoting survival of the granulocytic lineage cells and proliferation and migration of neutrophils as well as trophoblast cells. G-CSF acts by binding to its receptor G-CSFR (also called CSF3R), which after binding with G-CSF activates the canonical Janus kinase (Jak)/signal transducer, activator of transcription (STAT)and Ras/Raf/MAP kinase pathways. G-CSF potently stimulates the proliferation and release of peripheral blood progenitor cells into the bloodstream and is therefore used to treat neutropenia after chemotherapy. Furthermore, G-CSF levels are elevated upon intensive exercise leading to increased neutrophil counts, which are predominantly due to delayed neutrophil apoptosis. Specification Tested Reactivity: human Host / Isotype: Rabbit / IgG Class: Recombinant Type: Antibody Immunogen: CatNo: Product name: HumanKine® recombinant human G-CSF protein Source: -derived, Tag: Domain: of Sequence: Predict reactive species Full Name: colony stimulating factor 3 (granulocyte) Observed Molecular Weight: 20 kDa Gene Symbol: G-CSF Gene ID (NCBI): 1440 Conjugate: Unconjugated Form: Liquid Purification Method: Protein A purification UNIPROT ID: P09919 Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20 o C storage. 20ul sizes contain 0.1% BSA.